Mast cells and Kurloff cells - Their detection throughout the oestrous cycle in normal guinea pig ovaries and in guinea pigs with cystic rete ovarii

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F21%3A43879139" target="_blank" >RIV/62157124:16170/21:43879139 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0034528821001028" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0034528821001028</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.rvsc.2021.04.003" target="_blank" >10.1016/j.rvsc.2021.04.003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Mast cells and Kurloff cells - Their detection throughout the oestrous cycle in normal guinea pig ovaries and in guinea pigs with cystic rete ovarii

Popis výsledku v původním jazyce

Mast cells (MCs) and Kurloff cells (KCs) were detected in guinea pig ovaries in the follicular and luteal phases of the oestrous cycle. The samples of ovaries were fixed in Mota&apos;s basic lead acetate. Toluidine blue was used for detection of MCs and periodic acid-Schiff for detection of KCs. The percentage of KCs in a differential leukocyte count was determined in blood smears stained according to the Pappenheim method. Non-pregnant females with normal ovaries and with cystic rete ovarii were included in the study and the numbers of MCs and KCs were compared in these two groups and in follicular and luteal phases of the oestrous cycle. MCs&apos; distribution in ovaries was different in the guinea pig in comparison to previously studied species: MCs were found exclusively in the superficial layers of cortical stroma and no significant difference was found between the number of MCs in the follicular and luteal phases, neither in normal ovaries, nor in ovaries with cystic rete ovarii. Significantly lower numbers of MCs were found in ovaries with cystic rete ovarii (P &lt; 0.01) in contrast to normal ovaries. A significantly higher percentage of KCs in the peripheral blood was found in the follicular phase (P &lt; 0.05), whereas no significant difference was found in relation to the presence of cystic rete ovarii. Interestingly, no KCs were found in the samples of ovaries (either in the follicular or luteal phase, and with or without cysts). Thus, the expected role of KCs in ovarian physiology or in the aetiology of the cystic rete ovarii can be excluded.

Název v anglickém jazyce

Mast cells and Kurloff cells - Their detection throughout the oestrous cycle in normal guinea pig ovaries and in guinea pigs with cystic rete ovarii

Popis výsledku anglicky

Mast cells (MCs) and Kurloff cells (KCs) were detected in guinea pig ovaries in the follicular and luteal phases of the oestrous cycle. The samples of ovaries were fixed in Mota&apos;s basic lead acetate. Toluidine blue was used for detection of MCs and periodic acid-Schiff for detection of KCs. The percentage of KCs in a differential leukocyte count was determined in blood smears stained according to the Pappenheim method. Non-pregnant females with normal ovaries and with cystic rete ovarii were included in the study and the numbers of MCs and KCs were compared in these two groups and in follicular and luteal phases of the oestrous cycle. MCs&apos; distribution in ovaries was different in the guinea pig in comparison to previously studied species: MCs were found exclusively in the superficial layers of cortical stroma and no significant difference was found between the number of MCs in the follicular and luteal phases, neither in normal ovaries, nor in ovaries with cystic rete ovarii. Significantly lower numbers of MCs were found in ovaries with cystic rete ovarii (P &lt; 0.01) in contrast to normal ovaries. A significantly higher percentage of KCs in the peripheral blood was found in the follicular phase (P &lt; 0.05), whereas no significant difference was found in relation to the presence of cystic rete ovarii. Interestingly, no KCs were found in the samples of ovaries (either in the follicular or luteal phase, and with or without cysts). Thus, the expected role of KCs in ovarian physiology or in the aetiology of the cystic rete ovarii can be excluded.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Research in Veterinary Science

ISSN

0034-5288

e-ISSN

—

Svazek periodika

136

Číslo periodika v rámci svazku

May

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

512-518

Kód UT WoS článku

000694001000021

EID výsledku v databázi Scopus

2-s2.0-85104314938