Genotyping Toxoplasma gondii: whole genome sequences of isolates across Europe reveal diversity within European type II strains

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F22%3A43880129" target="_blank" >RIV/62157124:16170/22:43880129 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16170/22:43880147 RIV/62157124:16810/22:43880147

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Genotyping Toxoplasma gondii: whole genome sequences of isolates across Europe reveal diversity within European type II strains

Popis výsledku v původním jazyce

Toxoplasma gondii is a zoonotic protozoan parasite with an apparently largely clonal population structure in Europe. Few fine-scale typing methods exist for T. gondii. Whole genome sequence analysis of T. gondii offers new opportunities for the development of new discriminatory genotyping methods possibly with higher resolution and throughput for this parasite. We assessed the genomes of more than 70 T. gondii Type II isolates, including sequences of more than 60 European field isolates, by whole genome sequencing (WGS) and compared to the Type II reference strain Me49. Comparative analyses revealed up to four separate clusters of European type II T. gondii. The spatial distance between sites of isolation partially explained the detected genetic distance between isolates. We conclude that European T. gondii type II isolates are indeed genetically diverse. Based on our experiences, WGS may be useful to better understand the molecular epidemiology of the parasite in Europe, to trace infection sources in outbreaks and to detect the introduction of exotic or the emergence of recombinant strains.

Název v anglickém jazyce

Genotyping Toxoplasma gondii: whole genome sequences of isolates across Europe reveal diversity within European type II strains

Popis výsledku anglicky

Toxoplasma gondii is a zoonotic protozoan parasite with an apparently largely clonal population structure in Europe. Few fine-scale typing methods exist for T. gondii. Whole genome sequence analysis of T. gondii offers new opportunities for the development of new discriminatory genotyping methods possibly with higher resolution and throughput for this parasite. We assessed the genomes of more than 70 T. gondii Type II isolates, including sequences of more than 60 European field isolates, by whole genome sequencing (WGS) and compared to the Type II reference strain Me49. Comparative analyses revealed up to four separate clusters of European type II T. gondii. The spatial distance between sites of isolation partially explained the detected genetic distance between isolates. We conclude that European T. gondii type II isolates are indeed genetically diverse. Based on our experiences, WGS may be useful to better understand the molecular epidemiology of the parasite in Europe, to trace infection sources in outbreaks and to detect the introduction of exotic or the emergence of recombinant strains.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů