Genome-wide single nucleotide variation in Toxoplasma gondii type II isolates from Europe

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16810%2F21%3A43879377" target="_blank" >RIV/62157124:16810/21:43879377 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16170/21:43879377

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Genome-wide single nucleotide variation in Toxoplasma gondii type II isolates from Europe

Popis výsledku v původním jazyce

Background. Toxoplasma gondii is a highly prevalent protozoan parasite that globally infects a broad range of animals, including humans. A better knowledge of the genetic diversity and population structure of T. gondii may help to understand the many transmission routes and sources of infection. There are limited data on genome-wide comparisons of field isolates belonging to the same genotype or lineage. Therefore, the aim of the present study was to assess genome-wide genetic diversity among T. gondii type II isolates from Europe, where this lineage appears predominant. Material and Methods. Whole genome sequences of 4 European type II field isolates were assessed by whole genome sequencing (WGS) and highly polymorphic regions identified. These regions showed a considerable number of single nucleotide polymorphisms (SNPs), insertions and deletions (INDELS) relative to a T. gondii reference genome (strain ME49), available in a public data base (ToxoDB). Results. At least 95 % of the reads for each T. gondii European field isolate were mapped to the reference genome. The mapped reads covered over 99 % of the type II reference genome with a read depth of &gt;20 per base. The total number of SNPs varied between ~4000 and ~11000. Conclusion. This study demonstrates considerable genetic variation among European type II isolates and provides new insights into the population structure of T. gondii in Europe.

Název v anglickém jazyce

Genome-wide single nucleotide variation in Toxoplasma gondii type II isolates from Europe

Popis výsledku anglicky

Background. Toxoplasma gondii is a highly prevalent protozoan parasite that globally infects a broad range of animals, including humans. A better knowledge of the genetic diversity and population structure of T. gondii may help to understand the many transmission routes and sources of infection. There are limited data on genome-wide comparisons of field isolates belonging to the same genotype or lineage. Therefore, the aim of the present study was to assess genome-wide genetic diversity among T. gondii type II isolates from Europe, where this lineage appears predominant. Material and Methods. Whole genome sequences of 4 European type II field isolates were assessed by whole genome sequencing (WGS) and highly polymorphic regions identified. These regions showed a considerable number of single nucleotide polymorphisms (SNPs), insertions and deletions (INDELS) relative to a T. gondii reference genome (strain ME49), available in a public data base (ToxoDB). Results. At least 95 % of the reads for each T. gondii European field isolate were mapped to the reference genome. The mapped reads covered over 99 % of the type II reference genome with a read depth of &gt;20 per base. The total number of SNPs varied between ~4000 and ~11000. Conclusion. This study demonstrates considerable genetic variation among European type II isolates and provides new insights into the population structure of T. gondii in Europe.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů