Typing of European Toxoplasma gondii type II strains by a novel Ion AmpliSeq-based technique

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F22%3A43880130" target="_blank" >RIV/62157124:16170/22:43880130 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16810/22:43880130

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Typing of European Toxoplasma gondii type II strains by a novel Ion AmpliSeq-based technique

Popis výsledku v původním jazyce

Toxoplasma gondii is a protozoan parasite with a largely clonal population structure in the northern hemisphere. The clonal type II lineage prevails in Europe. A genotyping method, based on 15 microsatellite (MS) regions, represents the referencing standard, being able to distinguish between clonal lineages of T. gondii as well as to differentiate strains within these lineages using MS fingerprinting markers. We aimed to establish a highly discriminatory next-generation sequencing (NGS)-based typing method for T. gondii able to differentiate closely-related type II strains, to trace back infection sources and to monitor for exotic (non-canonical) or recombinant T. gondii strains.

Název v anglickém jazyce

Typing of European Toxoplasma gondii type II strains by a novel Ion AmpliSeq-based technique

Popis výsledku anglicky

Toxoplasma gondii is a protozoan parasite with a largely clonal population structure in the northern hemisphere. The clonal type II lineage prevails in Europe. A genotyping method, based on 15 microsatellite (MS) regions, represents the referencing standard, being able to distinguish between clonal lineages of T. gondii as well as to differentiate strains within these lineages using MS fingerprinting markers. We aimed to establish a highly discriminatory next-generation sequencing (NGS)-based typing method for T. gondii able to differentiate closely-related type II strains, to trace back infection sources and to monitor for exotic (non-canonical) or recombinant T. gondii strains.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů