A novel Ion AMpliSeq-based typing method of Toxoplasma gondii reveals aN ExCELLENT typing resolution among European type II strains

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F22%3A43880143" target="_blank" >RIV/62157124:16170/22:43880143 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16810/22:43880143

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

A novel Ion AMpliSeq-based typing method of Toxoplasma gondii reveals aN ExCELLENT typing resolution among European type II strains

Popis výsledku v původním jazyce

Toxoplasma gondii has a clonal population structure in Europe. The currently available typing method, based on 15 microsatellite regions, has a limited ability to discriminate between different type II strains. We therefore aimed to establish a next-generation sequencing (NGS)-based method with a higher typing resolution.Methods: T. gondii isolates were collected from different parts of Europe and assessed by whole genome sequencing (WGS). Highly polymorphic regions (HPRs) were identified. After confirmation by Sanger sequencing, 18 HPRs were used to design a primer panel suitable to establish an Ion AmpliSeq-based typing tool.

Název v anglickém jazyce

A novel Ion AMpliSeq-based typing method of Toxoplasma gondii reveals aN ExCELLENT typing resolution among European type II strains

Popis výsledku anglicky

Toxoplasma gondii has a clonal population structure in Europe. The currently available typing method, based on 15 microsatellite regions, has a limited ability to discriminate between different type II strains. We therefore aimed to establish a next-generation sequencing (NGS)-based method with a higher typing resolution.Methods: T. gondii isolates were collected from different parts of Europe and assessed by whole genome sequencing (WGS). Highly polymorphic regions (HPRs) were identified. After confirmation by Sanger sequencing, 18 HPRs were used to design a primer panel suitable to establish an Ion AmpliSeq-based typing tool.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů