Early administration of gramnegative gut anaerobes reduces E. coli colonization of day-old-chicks including a virulent APEC strain

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Early administration of gramnegative gut anaerobes reduces E. coli colonization of day-old-chicks including a virulent APEC strain

Popis výsledku v původním jazyce

High level of microbial contamination of newly hatched chicken is a risk factor for increased first-week mortality, massive use of antibiotic to reduce the losses a potentially transmission of high-risk virulent APEC clones throughout the production chain. Chicken hatched in commercial hatcheries, lacking a source of beneficial “adult” microflora, are prone to colonization by potential pathogens of the Enterobacteriaceae. Unlike common probiotics, administration of complex mixtures of anaerobic gut microflora proves to be more efficient in competitive exclusion of unwelcome bacteria and it is a promising means of reducing the microbial burden in broiler chicken. An experimental group of one-day-old chicken from a commercial hatchery was inoculated by a mixture of 7 gut anaerobes of known species. The next day the chicks of both experimental and control group were colonized by a ciprofloxacin-resistant APEC strain O78:H4-ST117. On days 7., 14., 21. and 28. 7 chicken of each group were euthanized, autopsied and serial dilutions of cecal contents cultivated on ciprofloxacin-supplemented Levine EMB agar. Specifically, the presence of the experimental strain was detected with an O78 antiserum.Significant reduction of colonization rates in the first two weeks was recorded in the experimental group for the numbers of ciprofloxacin-resistant E. coli. While the chicken of the control group showed 100% positivity for the experimental strain in first two weeks, in experimental chicken it remained under the detection level during the whole experiment. No differences in weight gain between groups were noted.The results show that colonization of chicken by defined anaerobic mixtures may provide a decisive protection during the critical period of the chicken intestinal microflora development and may reduce the need for antibiotic use in broiler chicken.

Název v anglickém jazyce

Early administration of gramnegative gut anaerobes reduces E. coli colonization of day-old-chicks including a virulent APEC strain

Popis výsledku anglicky

High level of microbial contamination of newly hatched chicken is a risk factor for increased first-week mortality, massive use of antibiotic to reduce the losses a potentially transmission of high-risk virulent APEC clones throughout the production chain. Chicken hatched in commercial hatcheries, lacking a source of beneficial “adult” microflora, are prone to colonization by potential pathogens of the Enterobacteriaceae. Unlike common probiotics, administration of complex mixtures of anaerobic gut microflora proves to be more efficient in competitive exclusion of unwelcome bacteria and it is a promising means of reducing the microbial burden in broiler chicken. An experimental group of one-day-old chicken from a commercial hatchery was inoculated by a mixture of 7 gut anaerobes of known species. The next day the chicks of both experimental and control group were colonized by a ciprofloxacin-resistant APEC strain O78:H4-ST117. On days 7., 14., 21. and 28. 7 chicken of each group were euthanized, autopsied and serial dilutions of cecal contents cultivated on ciprofloxacin-supplemented Levine EMB agar. Specifically, the presence of the experimental strain was detected with an O78 antiserum.Significant reduction of colonization rates in the first two weeks was recorded in the experimental group for the numbers of ciprofloxacin-resistant E. coli. While the chicken of the control group showed 100% positivity for the experimental strain in first two weeks, in experimental chicken it remained under the detection level during the whole experiment. No differences in weight gain between groups were noted.The results show that colonization of chicken by defined anaerobic mixtures may provide a decisive protection during the critical period of the chicken intestinal microflora development and may reduce the need for antibiotic use in broiler chicken.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů