Comparative genomic analysis of ESBL/AmpC Betalactamase-Producing E. coli from Czech Diary Calves

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F24%3A43881640" target="_blank" >RIV/62157124:16170/24:43881640 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16810/24:43881640

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparative genomic analysis of ESBL/AmpC Betalactamase-Producing E. coli from Czech Diary Calves

Popis výsledku v původním jazyce

This study analyzed 68 cefotaxime-resistant Escherichia coli isolates from calves on 16 Czech dairy farms and 15 isolates from farm workers to assess sequence types (STs), resistance and virulence genes, and zoonotic risks. Whole-genome sequencing (WGS) and bioinformatics tools were used for genetic characterization and phylogenetic analysis. Most calf isolates were multidrug-resistant, predominantly encoding blaCTX-M-14 (44.1%), blaCTX-M-1 (33.8%), blaCTX-M-8 (4.4%), and blaCTX-M-15 (16.2%). They harbored diverse plasmids (IncF, IncI1, IncB/O/K/Z, IncY) and primarily belonged to phylogenetic group A (51.5%), followed by B1 (23.5%), with lower representation of groups C, D, G, and E. One isolate carrying the IncI2-associated mcr-1 gene was detected. ST10 was the dominant sequence type (26.5%), with a zoonotic cluster (ST10-O101:H9) found in both calves and a farm worker. Another shared cluster involved ST34-O68:H30. Several isolates were potential zoonotic pathogens, including ExPEC (ST117, ST88, ST69) and STEC/EPEC strains carrying stx1, stx2, and/or the locus of enterocyte effacement (LEE). While genetic diversity was observed among calves from the same farm, some farms were dominated by clonal clusters. The observed strain diversity and gene combinations highlight the complexity of E. coli evolution and transmission dynamics. The presence of shared strains between calves and farm personnel further supports the risk of zoonotic transmission.

Název v anglickém jazyce

Comparative genomic analysis of ESBL/AmpC Betalactamase-Producing E. coli from Czech Diary Calves

Popis výsledku anglicky

This study analyzed 68 cefotaxime-resistant Escherichia coli isolates from calves on 16 Czech dairy farms and 15 isolates from farm workers to assess sequence types (STs), resistance and virulence genes, and zoonotic risks. Whole-genome sequencing (WGS) and bioinformatics tools were used for genetic characterization and phylogenetic analysis. Most calf isolates were multidrug-resistant, predominantly encoding blaCTX-M-14 (44.1%), blaCTX-M-1 (33.8%), blaCTX-M-8 (4.4%), and blaCTX-M-15 (16.2%). They harbored diverse plasmids (IncF, IncI1, IncB/O/K/Z, IncY) and primarily belonged to phylogenetic group A (51.5%), followed by B1 (23.5%), with lower representation of groups C, D, G, and E. One isolate carrying the IncI2-associated mcr-1 gene was detected. ST10 was the dominant sequence type (26.5%), with a zoonotic cluster (ST10-O101:H9) found in both calves and a farm worker. Another shared cluster involved ST34-O68:H30. Several isolates were potential zoonotic pathogens, including ExPEC (ST117, ST88, ST69) and STEC/EPEC strains carrying stx1, stx2, and/or the locus of enterocyte effacement (LEE). While genetic diversity was observed among calves from the same farm, some farms were dominated by clonal clusters. The observed strain diversity and gene combinations highlight the complexity of E. coli evolution and transmission dynamics. The presence of shared strains between calves and farm personnel further supports the risk of zoonotic transmission.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů