Comparison of two methods of measuring the urinary protein concentration for the determination of the urinary protein to creatinine ratio in various animal species

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F24%3A43881722" target="_blank" >RIV/62157124:16170/24:43881722 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16270/24:43881722

Výsledek na webu

<a href="https://vetmed.agriculturejournals.cz/artkey/vet-202408-0003_comparison-of-two-methods-of-measuring-the-urinary-protein-concentration-for-the-determination-of-the-urinary-p.php" target="_blank" >https://vetmed.agriculturejournals.cz/artkey/vet-202408-0003_comparison-of-two-methods-of-measuring-the-urinary-protein-concentration-for-the-determination-of-the-urinary-p.php</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.17221/30/2024-VETMED" target="_blank" >10.17221/30/2024-VETMED</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of two methods of measuring the urinary protein concentration for the determination of the urinary protein to creatinine ratio in various animal species

Popis výsledku v původním jazyce

Determination of the urinary protein-to-creatinine ratio (UPC) is an important tool in the quantification of proteinuria in animals. However, the result may be affected by the different methods of determining the urinary protein concentration. The aim of this study was to compare the turbidimetric method using benzethonium chloride and the colorimetric method using pyrogallol red in the measurement of the urinary protein concentration in dogs, cats, guinea pigs and horses. A total of 464, 192, 216 and 119 urine samples from dogs, cats, guinea pigs and horses were examined in the study, respectively. The group consisted of animals of both sexes and different ages, and, in the dogs and cats, it included both healthy animals and those with various health problems. In the group of horses and guinea pigs, only clinically healthy animals were included. A total of 347, 185, 103 and 100 samples from the dogs, cats, guinea pigs and horses were used in the statistical analysis; the other values were excluded as they were below the detection limit. According to the Passing-Bablok analysis, there was a significant constant and proportional difference in the horses. In the dogs, cats and guinea pigs, there was a significant constant difference, but no proportional difference. The Bland-Altman method showed significant bias between the two methods in the horses and cats, but not in the dogs and guinea pigs. In the dogs and cats, the agreement between the two methods was tested and expressed as Cohen&apos;s kappa (x). In the cats, it was almost perfect for the proteinuric samples (x = 0.823 3) and significant for the non-proteinuric samples (x = 0.804 9). In the dogs, the agreement was significant for the non-proteinuric samples (x = 0.621 5) and only moderate for the proteinuric samples (x = 0.527 5). The influence of the method used to determine the urinary protein concentration should be taken into account when evaluating the UPC. Repeated examinations in one patient should be performed with the same method.

Název v anglickém jazyce

Comparison of two methods of measuring the urinary protein concentration for the determination of the urinary protein to creatinine ratio in various animal species

Popis výsledku anglicky

Determination of the urinary protein-to-creatinine ratio (UPC) is an important tool in the quantification of proteinuria in animals. However, the result may be affected by the different methods of determining the urinary protein concentration. The aim of this study was to compare the turbidimetric method using benzethonium chloride and the colorimetric method using pyrogallol red in the measurement of the urinary protein concentration in dogs, cats, guinea pigs and horses. A total of 464, 192, 216 and 119 urine samples from dogs, cats, guinea pigs and horses were examined in the study, respectively. The group consisted of animals of both sexes and different ages, and, in the dogs and cats, it included both healthy animals and those with various health problems. In the group of horses and guinea pigs, only clinically healthy animals were included. A total of 347, 185, 103 and 100 samples from the dogs, cats, guinea pigs and horses were used in the statistical analysis; the other values were excluded as they were below the detection limit. According to the Passing-Bablok analysis, there was a significant constant and proportional difference in the horses. In the dogs, cats and guinea pigs, there was a significant constant difference, but no proportional difference. The Bland-Altman method showed significant bias between the two methods in the horses and cats, but not in the dogs and guinea pigs. In the dogs and cats, the agreement between the two methods was tested and expressed as Cohen&apos;s kappa (x). In the cats, it was almost perfect for the proteinuric samples (x = 0.823 3) and significant for the non-proteinuric samples (x = 0.804 9). In the dogs, the agreement was significant for the non-proteinuric samples (x = 0.621 5) and only moderate for the proteinuric samples (x = 0.527 5). The influence of the method used to determine the urinary protein concentration should be taken into account when evaluating the UPC. Repeated examinations in one patient should be performed with the same method.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Veterinární medicína

ISSN

0375-8427

e-ISSN

1805-9392

Svazek periodika

69

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

273-280

Kód UT WoS článku

001306017500002

EID výsledku v databázi Scopus

—