Electrochemical determination of metallothionein in the domestic fowl

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F09%3A00002133" target="_blank" >RIV/62157124:16270/09:00002133 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical determination of metallothionein in the domestic fowl

Popis výsledku v původním jazyce

Metallothionein (MT) belongs to group of intracellular, low-molecular and cysteine-rich proteins with a molecular weight from 6 to 10 kDa. Owing to their high affinity to heavy metals (Zn, Cd, As, etc.) their main role is homeostatic control and detoxification of metal ions in an organism. In the present work we aimed at suggesting and utilizing electroanalytical techniques to determine content of MT in the blood serum of domestic fowls. Electrochemical measurements were performed with an AUTOLAB Analyser connected to VA-Stand 663, using a standard cell with three electrodes. Particularly, MT was detected by adsorptive transfer stripping technique in connection with differential pulse voltammetry. The detection limit of MT was estimated down to 100 fM(standards only) or down to 100 pM measured in the presence of blood serum. The average content of MT was 21.3 ?M. The MT level in hens was about 25 % higher than in cocks. This phenomenon can be related to higher demands on the content o

Název v anglickém jazyce

Electrochemical determination of metallothionein in the domestic fowl

Popis výsledku anglicky

Metallothionein (MT) belongs to group of intracellular, low-molecular and cysteine-rich proteins with a molecular weight from 6 to 10 kDa. Owing to their high affinity to heavy metals (Zn, Cd, As, etc.) their main role is homeostatic control and detoxification of metal ions in an organism. In the present work we aimed at suggesting and utilizing electroanalytical techniques to determine content of MT in the blood serum of domestic fowls. Electrochemical measurements were performed with an AUTOLAB Analyser connected to VA-Stand 663, using a standard cell with three electrodes. Particularly, MT was detected by adsorptive transfer stripping technique in connection with differential pulse voltammetry. The detection limit of MT was estimated down to 100 fM(standards only) or down to 100 pM measured in the presence of blood serum. The average content of MT was 21.3 ?M. The MT level in hens was about 25 % higher than in cocks. This phenomenon can be related to higher demands on the content o

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Natura Croatica

ISSN

1330-0520

e-ISSN

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Svazek periodika

17

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

HR - Chorvatská republika

Počet stran výsledku

10

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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