LC-MS analyses of microcystins in fish tissues overestimate toxin levels-critical comparison with LS-MS/MS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F10%3A00002752" target="_blank" >RIV/62157124:16270/10:00002752 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

LC-MS analyses of microcystins in fish tissues overestimate toxin levels-critical comparison with LS-MS/MS

Popis výsledku v původním jazyce

Microcystins are cyclic peptide toxins with hepatotoxic and tumour-promoting properties which are produced in high quantities in freshwater cyanobacterial water blooms, and several studies have reported microcystin accumulation in fish with possible foodtransfer to humans. In this study, we provide the first comparison of liquid chromatography with single mass-spectrometric and with tandem massspectrometric detection for analyses of microcystins in complex fish tissue samples. Use of traditional singlemass spectrometry (i.e. monitoring of ions with m/z 519.5 for microcystin-RR and m/z 995.5 for microcystin-LR) was found to provide false-positive responses, thus overestimating the concentrations of microcystins in the tissue samples.More selective tandem mass spectrometry seems to provide more reliable results. The concentrations of microcystins detected by tandem mass spectrometry in fish from controlled exposure experiments were more than 50% Loir in comparison with concentrations o

Název v anglickém jazyce

LC-MS analyses of microcystins in fish tissues overestimate toxin levels-critical comparison with LS-MS/MS

Popis výsledku anglicky

Microcystins are cyclic peptide toxins with hepatotoxic and tumour-promoting properties which are produced in high quantities in freshwater cyanobacterial water blooms, and several studies have reported microcystin accumulation in fish with possible foodtransfer to humans. In this study, we provide the first comparison of liquid chromatography with single mass-spectrometric and with tandem massspectrometric detection for analyses of microcystins in complex fish tissue samples. Use of traditional singlemass spectrometry (i.e. monitoring of ions with m/z 519.5 for microcystin-RR and m/z 995.5 for microcystin-LR) was found to provide false-positive responses, thus overestimating the concentrations of microcystins in the tissue samples.More selective tandem mass spectrometry seems to provide more reliable results. The concentrations of microcystins detected by tandem mass spectrometry in fish from controlled exposure experiments were more than 50% Loir in comparison with concentrations o

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)<br>V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

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Svazek periodika

398

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000281670400008

EID výsledku v databázi Scopus

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