The effects of subchronic exposure to ciprofloxacin on zebrafish (Danio rerio)

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F14%3A43872883" target="_blank" >RIV/62157124:16270/14:43872883 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16170/14:43872883

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The effects of subchronic exposure to ciprofloxacin on zebrafish (Danio rerio)

Popis výsledku v původním jazyce

OBJECTIVES: The aim of this study was to assess the impact of ciprofloxacin in Danio rerio on growth, on the development of histopathological changes in selected organs (gills, kidney, liver), and on the activity of some oxidative stress markers during a 28-day toxicity test. METHODS: Juvenile growth tests were performed on D. rerio according to OECD guideline No. 215. Fish at the age of 30 days were exposed for 28 days to a range of concentrations of ciprofloxacin (0.7 ?g.L-1 - environmental concentration, 100, 650, 1 100 and 3 000 ?g.L-1). RESULTS: There were no significant differences between the specific growth rates of fish from the test groups and from the control group. Histopathological examination revealed no pathological changes in organs of fish exposed to any ciprofloxacin concentration. The activity of glutathione S-transferase increased at the ciprofloxacin concentrations of 0.7 and 100 ?g.L-1 compared to the control. A significant decrease in glutathione reductase activity was obtained in fish exposed to ciprofloxacin at 1 100 and 3 000 ?g.L-1; a significant decrease in glutathione peroxidase activity was also found, but at all tested concentrations except for 100 ?g.L-1. A decrease in the concentration of thiobarbituric acid reactive sub-stances was noted only at 100 ?g.L-1 compared to the control. CONCLUSIONS: According to our results, all tested concentrations of ciprofloxacin, including the environmental concentration, had an influence on oxidative stress markers and detoxifying enzymes in exposed fish, but did not affect fish growth or cause the development of histopathological changes in the fish organism.

Název v anglickém jazyce

The effects of subchronic exposure to ciprofloxacin on zebrafish (Danio rerio)

Popis výsledku anglicky

OBJECTIVES: The aim of this study was to assess the impact of ciprofloxacin in Danio rerio on growth, on the development of histopathological changes in selected organs (gills, kidney, liver), and on the activity of some oxidative stress markers during a 28-day toxicity test. METHODS: Juvenile growth tests were performed on D. rerio according to OECD guideline No. 215. Fish at the age of 30 days were exposed for 28 days to a range of concentrations of ciprofloxacin (0.7 ?g.L-1 - environmental concentration, 100, 650, 1 100 and 3 000 ?g.L-1). RESULTS: There were no significant differences between the specific growth rates of fish from the test groups and from the control group. Histopathological examination revealed no pathological changes in organs of fish exposed to any ciprofloxacin concentration. The activity of glutathione S-transferase increased at the ciprofloxacin concentrations of 0.7 and 100 ?g.L-1 compared to the control. A significant decrease in glutathione reductase activity was obtained in fish exposed to ciprofloxacin at 1 100 and 3 000 ?g.L-1; a significant decrease in glutathione peroxidase activity was also found, but at all tested concentrations except for 100 ?g.L-1. A decrease in the concentration of thiobarbituric acid reactive sub-stances was noted only at 100 ?g.L-1 compared to the control. CONCLUSIONS: According to our results, all tested concentrations of ciprofloxacin, including the environmental concentration, had an influence on oxidative stress markers and detoxifying enzymes in exposed fish, but did not affect fish growth or cause the development of histopathological changes in the fish organism.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Neuroendocrinology Letters

ISSN

0172-780X

e-ISSN

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Svazek periodika

35

Číslo periodika v rámci svazku

S2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

64-70

Kód UT WoS článku

000351064900008

EID výsledku v databázi Scopus

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