Predilection sites for Toxoplasma gondii in sheep tissues revealed by magnetic capture and real-time PCR detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F15%3A43873359" target="_blank" >RIV/62157124:16270/15:43873359 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16170/15:43873359 RIV/62157124:16810/15:43873359

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.fm.2015.07.005" target="_blank" >http://dx.doi.org/10.1016/j.fm.2015.07.005</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fm.2015.07.005" target="_blank" >10.1016/j.fm.2015.07.005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Predilection sites for Toxoplasma gondii in sheep tissues revealed by magnetic capture and real-time PCR detection

Popis výsledku v původním jazyce

Undercooked lamb and mutton are common sources of Toxoplasma gondii infection for humans. A sequence specific magnetic capture technique in combination with quantitative real-time PCR targeting the 529 bp repeat element of T. gondii was used for estimation of the parasite burdens in various sheep tissues (n = 6) three months after peroral experimental inoculation with 10,000 T. gondii oocysts. Brain was the most frequently affected organ (positive in all 6 sheep) and showed the highest estimated parasite loads (0.5-30,913 parasites/g tissue). Lung samples were positive in three sheep, with load estimates of 36.3 to < 1 parasite/g tissue. Heart tissue was positive in three sheep and kidney only in one animal with low parasite loads (< 1 parasite/g tissue). Only few skeletal muscle samples in 2 animals showed positive results, with very low parasite burdens, while samples from further internal organs (i.e. liver and spleen) were negative in all animals. This study identified the brain as

Název v anglickém jazyce

Predilection sites for Toxoplasma gondii in sheep tissues revealed by magnetic capture and real-time PCR detection

Popis výsledku anglicky

Undercooked lamb and mutton are common sources of Toxoplasma gondii infection for humans. A sequence specific magnetic capture technique in combination with quantitative real-time PCR targeting the 529 bp repeat element of T. gondii was used for estimation of the parasite burdens in various sheep tissues (n = 6) three months after peroral experimental inoculation with 10,000 T. gondii oocysts. Brain was the most frequently affected organ (positive in all 6 sheep) and showed the highest estimated parasite loads (0.5-30,913 parasites/g tissue). Lung samples were positive in three sheep, with load estimates of 36.3 to < 1 parasite/g tissue. Heart tissue was positive in three sheep and kidney only in one animal with low parasite loads (< 1 parasite/g tissue). Only few skeletal muscle samples in 2 animals showed positive results, with very low parasite burdens, while samples from further internal organs (i.e. liver and spleen) were negative in all animals. This study identified the brain as

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GJ - Choroby a škůdci zvířat, veterinární medicina

OECD FORD obor

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Projekt

<a href="/cs/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: CEITEC - central european institute of technology</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food Microbiology

ISSN

0740-0020

e-ISSN

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Svazek periodika

52

Číslo periodika v rámci svazku

December

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

4

Strana od-do

150-153

Kód UT WoS článku

000360653500019

EID výsledku v databázi Scopus

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