Comparison of Toxoplasma gondii distribution in tissues of experimentally infected pigs

Kód výsledku v IS VaVaI

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RIV/62157124:16170/22:43880132 RIV/62157124:16810/22:43880132 RIV/62157124:16170/23:43880656

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of Toxoplasma gondii distribution in tissues of experimentally infected pigs

Popis výsledku v původním jazyce

Toxoplasma gondii is a food-borne zoonotic parasite capable of infecting essentially all warmblooded animals, including humans. With a majority of infections in Europe estimated to be meat-borne, pork, represents a potential risk for consumers. Therefore, we aimed to investigate and evaluate the anatomical distribution of T. gondii within the tissues of experimentally infected pigs and to identify the predilection sites for the different T. gondii isolates and the two infectious stages used. A selected set of samples, representing the commonly consumed meat cuts (n=4) and organs (n=5), were collected from each of the total of 22 pigs. Animals were divided into four groups, with each group inoculated orally with a different combination of T. gondii isolates (type II or III) and sources of infection (400 oocysts or 10 tissue cysts). In the four complete sets of samples selected for the testing, DNA of T. gondii was confirmed by qPCR in 27.2 % (6/22) hearts, 54.5 % (12/22) loins, 54.5 % (12/22) hams and 50.0 % (11/22) shoulder samples. Animals infected with T. gondii oocysts proved to be positive for T. gondii DNA by qPCR in 25 % (10/40) of cases, compared to 64.6 % (31/48) of those infected with T. gondii tissue cysts. Infection with T. gondii type II resulted in 22.9 % (11/48) positives by qPCR and 75 % (30/40) in pigs infected with T. gondii type III. Results suggest an uneven concentration of cysts within the tissues of the tested animals, with an unexpectedly low prevalence in hearts. Differences between the infection with oocyst and tissue cyst stages, as well as between the two different isolates of T. gondii parasites used in this study, provide an interesting comparison of distribution patterns of T. gondii based on the source of infection.

Název v anglickém jazyce

Comparison of Toxoplasma gondii distribution in tissues of experimentally infected pigs

Popis výsledku anglicky

Toxoplasma gondii is a food-borne zoonotic parasite capable of infecting essentially all warmblooded animals, including humans. With a majority of infections in Europe estimated to be meat-borne, pork, represents a potential risk for consumers. Therefore, we aimed to investigate and evaluate the anatomical distribution of T. gondii within the tissues of experimentally infected pigs and to identify the predilection sites for the different T. gondii isolates and the two infectious stages used. A selected set of samples, representing the commonly consumed meat cuts (n=4) and organs (n=5), were collected from each of the total of 22 pigs. Animals were divided into four groups, with each group inoculated orally with a different combination of T. gondii isolates (type II or III) and sources of infection (400 oocysts or 10 tissue cysts). In the four complete sets of samples selected for the testing, DNA of T. gondii was confirmed by qPCR in 27.2 % (6/22) hearts, 54.5 % (12/22) loins, 54.5 % (12/22) hams and 50.0 % (11/22) shoulder samples. Animals infected with T. gondii oocysts proved to be positive for T. gondii DNA by qPCR in 25 % (10/40) of cases, compared to 64.6 % (31/48) of those infected with T. gondii tissue cysts. Infection with T. gondii type II resulted in 22.9 % (11/48) positives by qPCR and 75 % (30/40) in pigs infected with T. gondii type III. Results suggest an uneven concentration of cysts within the tissues of the tested animals, with an unexpectedly low prevalence in hearts. Differences between the infection with oocyst and tissue cyst stages, as well as between the two different isolates of T. gondii parasites used in this study, provide an interesting comparison of distribution patterns of T. gondii based on the source of infection.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů