TROPISM OF TOXOPLASMA GONDII IN THE TISSUES OF EXPERIMENTALLY INFECTED PIGS

Kód výsledku v IS VaVaI

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RIV/62157124:16170/21:43879378

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

TROPISM OF TOXOPLASMA GONDII IN THE TISSUES OF EXPERIMENTALLY INFECTED PIGS

Popis výsledku v původním jazyce

Background. Toxoplasma gondii is a food-borne zoonotic parasite capable of infecting essentially all warmblooded animals, including humans. With a majority of infections in Europe estimated to be meat-borne, pork, as one of the most consumed meats in the Czech Republic, represents a potential risk for consumers. Therefore, we aimed to investigate and evaluate the anatomical distribution of T. gondiiwithin the tissues of experimentally infected pigs and to identify the predilection sites for the different T. gondii isolates and the two infectious stages used. Material and Methods. A selected set of samples, representing 4 commonly consumed meat cuts and organs were collected from each of the total of 22 pigs. Animals were divided into 4 groups, with each group inoculated orally with a different combination of T. gondii isolates (genotype II or III) and sources of infection (400 oocysts or 10 tissue cysts). Results. In the four complete sets of samples selected for the testing, DNA of T. gondii was confirmed by qPCR in 27.2 % (6/22) hearts, 54.5 % (12/22) loins, 54.5 % (12/22) hams and 50.0 % (11/22) shoulder samples. Animals infected by oocysts proved to be positive for T. gondiiby qPCR in 25 % (10/40) of cases, compared to 64.6 % (31/48) of those infected by tissue cysts. Infection by genotype II and III resulted in 22.9 % (11/48) and 75 % (30/40) T. gondii positives by qPCR respectively. Conclusion. Results suggest an uneven concentration of cysts within the tissues of the tested animals, with an unexpectedly low prevalence in hearts. Differences between the infection with oocyst and tissue cyst stages, as well as between the two different isolates of T. gondii parasites used in this study, provide an interesting comparison of distribution patterns of T. gondii based on the source of infection.

Název v anglickém jazyce

TROPISM OF TOXOPLASMA GONDII IN THE TISSUES OF EXPERIMENTALLY INFECTED PIGS

Popis výsledku anglicky

Background. Toxoplasma gondii is a food-borne zoonotic parasite capable of infecting essentially all warmblooded animals, including humans. With a majority of infections in Europe estimated to be meat-borne, pork, as one of the most consumed meats in the Czech Republic, represents a potential risk for consumers. Therefore, we aimed to investigate and evaluate the anatomical distribution of T. gondiiwithin the tissues of experimentally infected pigs and to identify the predilection sites for the different T. gondii isolates and the two infectious stages used. Material and Methods. A selected set of samples, representing 4 commonly consumed meat cuts and organs were collected from each of the total of 22 pigs. Animals were divided into 4 groups, with each group inoculated orally with a different combination of T. gondii isolates (genotype II or III) and sources of infection (400 oocysts or 10 tissue cysts). Results. In the four complete sets of samples selected for the testing, DNA of T. gondii was confirmed by qPCR in 27.2 % (6/22) hearts, 54.5 % (12/22) loins, 54.5 % (12/22) hams and 50.0 % (11/22) shoulder samples. Animals infected by oocysts proved to be positive for T. gondiiby qPCR in 25 % (10/40) of cases, compared to 64.6 % (31/48) of those infected by tissue cysts. Infection by genotype II and III resulted in 22.9 % (11/48) and 75 % (30/40) T. gondii positives by qPCR respectively. Conclusion. Results suggest an uneven concentration of cysts within the tissues of the tested animals, with an unexpectedly low prevalence in hearts. Differences between the infection with oocyst and tissue cyst stages, as well as between the two different isolates of T. gondii parasites used in this study, provide an interesting comparison of distribution patterns of T. gondii based on the source of infection.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů