Comparison of Toxoplasma gondii distribution in tissues of experimentally infected pigs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16170%2F22%3A43880132" target="_blank" >RIV/62157124:16170/22:43880132 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62157124:16810/22:43880132 RIV/62157124:16170/23:43880656 RIV/62157124:16810/23:43880656

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of Toxoplasma gondii distribution in tissues of experimentally infected pigs

Popis výsledku v původním jazyce

Toxoplasma gondii is a protozoan zoonotic parasite with a worldwide distribution. In Europe, the majority of human infections are estimated to be food-borne, with meat of the infected animals playing an important role in the transmission. Pork is one of the most frequently consumed meats in Europe, and therefore represents a potential risk if consumed raw or undercooked. We aimed to investigate and evaluate the anatomical distribution of T. gondii within the tissues of experimentally infected pigs with various T. gondii isolates and infectious stages. A total of 22 pigs were divided in four groups based on the combination of different T. gondii isolates (type II or III) and infectious stages (400 oocysts or 10 tissue cysts) used during the experimental infection. A set of meat cuts (n=3) and organs (n=6), was collected from each pig, and investigated by means of qPCR. Proportion of meat cuts positive for T. gondii DNA was 50.0 % (11/22) in the shoulders, 54.5 % (12/22) in the loins, and 54.5 % (12/22) in the hams. Proportion of the organs positive for T. gondii DNA was 0.0 % (0/22) in the livers, 4.5 % (1/22) in the spleens, 13.6 % (3/22) in the lungs, 13.6 % (3/22) in the kidneys, 27.3 % (6/22) in the hearts, and 63.6 % (14/22) in the brains. Overall, T. gondii DNA was confirmed in 16.7 % (15/90) of the pigs infected with oocysts, and in 42.6 % (46/108) of the pigs infected with tissue cysts. Infection with type II resulted in 14.8 % (16/108) of positive tissues, and in 47.8 % (43/90) of positive tissues in the pigs infected with type III. The results suggest an uneven concentration of tissue cysts in the tested tissues. The differences between the infection with oocyst and tissue cyst stages, as well as between the two different isolates of T. gondii parasites used in this study, provide an interesting comparison of distribution patterns of T. gondii based on the source of infection.

Název v anglickém jazyce

Comparison of Toxoplasma gondii distribution in tissues of experimentally infected pigs

Popis výsledku anglicky

Toxoplasma gondii is a protozoan zoonotic parasite with a worldwide distribution. In Europe, the majority of human infections are estimated to be food-borne, with meat of the infected animals playing an important role in the transmission. Pork is one of the most frequently consumed meats in Europe, and therefore represents a potential risk if consumed raw or undercooked. We aimed to investigate and evaluate the anatomical distribution of T. gondii within the tissues of experimentally infected pigs with various T. gondii isolates and infectious stages. A total of 22 pigs were divided in four groups based on the combination of different T. gondii isolates (type II or III) and infectious stages (400 oocysts or 10 tissue cysts) used during the experimental infection. A set of meat cuts (n=3) and organs (n=6), was collected from each pig, and investigated by means of qPCR. Proportion of meat cuts positive for T. gondii DNA was 50.0 % (11/22) in the shoulders, 54.5 % (12/22) in the loins, and 54.5 % (12/22) in the hams. Proportion of the organs positive for T. gondii DNA was 0.0 % (0/22) in the livers, 4.5 % (1/22) in the spleens, 13.6 % (3/22) in the lungs, 13.6 % (3/22) in the kidneys, 27.3 % (6/22) in the hearts, and 63.6 % (14/22) in the brains. Overall, T. gondii DNA was confirmed in 16.7 % (15/90) of the pigs infected with oocysts, and in 42.6 % (46/108) of the pigs infected with tissue cysts. Infection with type II resulted in 14.8 % (16/108) of positive tissues, and in 47.8 % (43/90) of positive tissues in the pigs infected with type III. The results suggest an uneven concentration of tissue cysts in the tested tissues. The differences between the infection with oocyst and tissue cyst stages, as well as between the two different isolates of T. gondii parasites used in this study, provide an interesting comparison of distribution patterns of T. gondii based on the source of infection.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů