Antimicrobial-Resistant and Extended-Spectrum ?-Lactamase-Producing Escherichia coli in Raw Cow's Milk

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F15%3A43873614" target="_blank" >RIV/62157124:16270/15:43873614 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.4315/0362-028X.JFP-14-250" target="_blank" >http://dx.doi.org/10.4315/0362-028X.JFP-14-250</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4315/0362-028X.JFP-14-250" target="_blank" >10.4315/0362-028X.JFP-14-250</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Antimicrobial-Resistant and Extended-Spectrum ?-Lactamase-Producing Escherichia coli in Raw Cow's Milk

Popis výsledku v původním jazyce

The occurrence of antimicrobial-resistant bacteria is an important public health issue. The aim of this study was the monitoring of resistant Escherichia coli in raw cow's milk with a focus on the detection of extended-spectrum b-lactamase (ESBL)-producing strains. In total, 263 samples of raw milk from 40 farms were collected and investigated in 2010 to 2013 in the Czech Republic. Detection of E. coli was performed and evaluated according to ISO 16649-2, and antibiotik resistance was screened by the disk diffusion method. The presence of E. coli was detected in 243 (92.4%) samples. In total, 270 isolates were obtained. Resistance to b-lactam (31.8%) and tetracycline (13.0%) antibiotics was detected most often and also multiresistant strains (5.5%) were observed. E. coli isolates found to be resistant to b-lactam, tetracycline, and quinolone antibiotics were assayed by PCR to detect selected genes encoding those resistance mechanisms. In isolates in which any bla genes were detected, a

Název v anglickém jazyce

Antimicrobial-Resistant and Extended-Spectrum ?-Lactamase-Producing Escherichia coli in Raw Cow's Milk

Popis výsledku anglicky

The occurrence of antimicrobial-resistant bacteria is an important public health issue. The aim of this study was the monitoring of resistant Escherichia coli in raw cow's milk with a focus on the detection of extended-spectrum b-lactamase (ESBL)-producing strains. In total, 263 samples of raw milk from 40 farms were collected and investigated in 2010 to 2013 in the Czech Republic. Detection of E. coli was performed and evaluated according to ISO 16649-2, and antibiotik resistance was screened by the disk diffusion method. The presence of E. coli was detected in 243 (92.4%) samples. In total, 270 isolates were obtained. Resistance to b-lactam (31.8%) and tetracycline (13.0%) antibiotics was detected most often and also multiresistant strains (5.5%) were observed. E. coli isolates found to be resistant to b-lactam, tetracycline, and quinolone antibiotics were assayed by PCR to detect selected genes encoding those resistance mechanisms. In isolates in which any bla genes were detected, a

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

GM - Potravinářství

OECD FORD obor

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Projekt

<a href="/cs/project/QJ1230044" target="_blank" >QJ1230044: Stanovení parametrů pro legislativní hodnocení kvality a zdravotní nezávadnosti syrového mléka krav, ovcí a koz</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Food Protection

ISSN

0362-028X

e-ISSN

—

Svazek periodika

78

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

72-77

Kód UT WoS článku

000348012000010

EID výsledku v databázi Scopus

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