Detection of fluoroquinolone residues in milk using Yersinia spp. strains: towards better sensitivity for flumequine determination

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F17%3A43876204" target="_blank" >RIV/62157124:16270/17:43876204 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1515/jvetres-2017-0061" target="_blank" >http://dx.doi.org/10.1515/jvetres-2017-0061</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1515/jvetres-2017-0061" target="_blank" >10.1515/jvetres-2017-0061</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection of fluoroquinolone residues in milk using Yersinia spp. strains: towards better sensitivity for flumequine determination

Popis výsledku v původním jazyce

Plate diffusion methods play an important role in the monitoring system for antimicrobial agents in raw materials and foodstuffs of animal origin. The aim of this work was to select a Yersinia spp. strain for the plate diffusion method based on sensitivity to a fluoroquinolone, namely flumequine. Another aim was to determine the optimal conditions of the method with the selected strain of Yersinia ruckeri CCM 8467 and to determine the detection capability (CC?) of this method for residues of selected fluoroquinolones in milk. Material and Methods: Optimum method conditions were set: cell concentration in the test agar at the level of 9.105?106 CFU/mL, discs with a diameter of 12.7 mm, Antimicrobial Inhibitor Test Agar with a pH of 6.0, and incubation at 30°C for at least 18 h and up to 24 h. Results: With respect to the maximum residue limit (MRL), the Y. ruckeri plate method demonstrated the lowest sensitivity to flumequine. The CC? of the method for flumequine was in the concentration of 100 ?g/L (twice the MRL). The study also confirmed that the method exhibits very good sensitivity to the other tested fluoroquinolones, which were marbofloxacin (30 ?g/L, 0.4 MRL), ciprofloxacin (10 ?g/L, 0.1 MRL), and enrofloxacin (20 ?g/L, 0.2 MRL), but lower sensitivity to danofloxacin (42 ?g/L, 1.4 MRL). Conclusion: The method with the CCM 8467 strain of Y. ruckeri showed a higher sensitivity to flumequine than the method with the ATCC 11303 strain of E. coli.

Název v anglickém jazyce

Detection of fluoroquinolone residues in milk using Yersinia spp. strains: towards better sensitivity for flumequine determination

Popis výsledku anglicky

Plate diffusion methods play an important role in the monitoring system for antimicrobial agents in raw materials and foodstuffs of animal origin. The aim of this work was to select a Yersinia spp. strain for the plate diffusion method based on sensitivity to a fluoroquinolone, namely flumequine. Another aim was to determine the optimal conditions of the method with the selected strain of Yersinia ruckeri CCM 8467 and to determine the detection capability (CC?) of this method for residues of selected fluoroquinolones in milk. Material and Methods: Optimum method conditions were set: cell concentration in the test agar at the level of 9.105?106 CFU/mL, discs with a diameter of 12.7 mm, Antimicrobial Inhibitor Test Agar with a pH of 6.0, and incubation at 30°C for at least 18 h and up to 24 h. Results: With respect to the maximum residue limit (MRL), the Y. ruckeri plate method demonstrated the lowest sensitivity to flumequine. The CC? of the method for flumequine was in the concentration of 100 ?g/L (twice the MRL). The study also confirmed that the method exhibits very good sensitivity to the other tested fluoroquinolones, which were marbofloxacin (30 ?g/L, 0.4 MRL), ciprofloxacin (10 ?g/L, 0.1 MRL), and enrofloxacin (20 ?g/L, 0.2 MRL), but lower sensitivity to danofloxacin (42 ?g/L, 1.4 MRL). Conclusion: The method with the CCM 8467 strain of Y. ruckeri showed a higher sensitivity to flumequine than the method with the ATCC 11303 strain of E. coli.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/QJ1230044" target="_blank" >QJ1230044: Stanovení parametrů pro legislativní hodnocení kvality a zdravotní nezávadnosti syrového mléka krav, ovcí a koz</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Veterinary Research

ISSN

2450-7393

e-ISSN

—

Svazek periodika

61

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

6

Strana od-do

467-472

Kód UT WoS článku

000418995600014

EID výsledku v databázi Scopus

2-s2.0-85042924965