A microbiological multi-plate method to detect cephalosporin residues in milk

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F20%3A43878970" target="_blank" >RIV/62157124:16270/20:43878970 - isvavai.cz</a>

Výsledek na webu

<a href="https://actavet.vfu.cz/media/pdf/actavet_2020089020201.pdf" target="_blank" >https://actavet.vfu.cz/media/pdf/actavet_2020089020201.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb202089020201" target="_blank" >10.2754/avb202089020201</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A microbiological multi-plate method to detect cephalosporin residues in milk

Popis výsledku v původním jazyce

The aim of this study was to determine the detection capability (CC beta) of a multi-plate diffusion method for selected cephalosporins for which the maximum residue limits (MRLs) in milk have been set (ceftiofur, cefoperazone, cephalexine, cephazoline, cephalonium, cephapirine, cefquinome). The multiplate method was composed of Bacillus subtilis BGA CCM 4062 plates (agar pH of 6, 8 and 7.2); Kocuria rhizophila CCM 552 plate, Geobacillus stearothermophilus CCM 5965 plate and Escherichia coli CCM 7372 plate. Geobacillus stearothermophilus plate showed the highest sensitivity. CC beta for the tested cephalosporins was determined at MRLs or lower, except for cefquinome, which was undetectable even at 12 × the MRL. Kocuria rhizophila plate showed the highest sensitivity to ceftiofur (CC beta = 100 ug/l); cephazoline and cephalonium could not be detected even at 12 × times the MRL. Escherichia coli plate was most sensitive to cefoperazone (CC beta = 60 ug/l). Other than cephapirine, none of the cephalosporins could be detected by B. subtilis BGA (pH 6, 8 and 7.2) plates even at 12 × the MRL. Our results demonstrate the differences in sensitivity to selected cephalosporins of the individual plate methods that together form the multi-plate method. The multi-plate method is sensitive enough to detect most of the tested cephalosporins, with the exception of cefquinome, which could not be detected at levels close to MRL.

Název v anglickém jazyce

A microbiological multi-plate method to detect cephalosporin residues in milk

Popis výsledku anglicky

The aim of this study was to determine the detection capability (CC beta) of a multi-plate diffusion method for selected cephalosporins for which the maximum residue limits (MRLs) in milk have been set (ceftiofur, cefoperazone, cephalexine, cephazoline, cephalonium, cephapirine, cefquinome). The multiplate method was composed of Bacillus subtilis BGA CCM 4062 plates (agar pH of 6, 8 and 7.2); Kocuria rhizophila CCM 552 plate, Geobacillus stearothermophilus CCM 5965 plate and Escherichia coli CCM 7372 plate. Geobacillus stearothermophilus plate showed the highest sensitivity. CC beta for the tested cephalosporins was determined at MRLs or lower, except for cefquinome, which was undetectable even at 12 × the MRL. Kocuria rhizophila plate showed the highest sensitivity to ceftiofur (CC beta = 100 ug/l); cephazoline and cephalonium could not be detected even at 12 × times the MRL. Escherichia coli plate was most sensitive to cefoperazone (CC beta = 60 ug/l). Other than cephapirine, none of the cephalosporins could be detected by B. subtilis BGA (pH 6, 8 and 7.2) plates even at 12 × the MRL. Our results demonstrate the differences in sensitivity to selected cephalosporins of the individual plate methods that together form the multi-plate method. The multi-plate method is sensitive enough to detect most of the tested cephalosporins, with the exception of cefquinome, which could not be detected at levels close to MRL.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

—

Svazek periodika

89

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

8

Strana od-do

201-208

Kód UT WoS článku

000548359900012

EID výsledku v databázi Scopus

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