The effect of minor mycotoxin enniatin B on in vitro dry matter digestibility

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The effect of minor mycotoxin enniatin B on in vitro dry matter digestibility

Popis výsledku v původním jazyce

The aim of this study was to determine the effect of minor mycotoxin enniatin B (Sigma-Aldrich, dissolved in methanol) on dry matter digestibility of total mixed ration. The experiments were performed in vitro using a rumen fluid-buffer system. Rumen fluid was taken from dairy cows fed on diet consisted of maize silage (22 kg), lucerne haylage (12 kg), meadow hay (3 kg), feeding straw (1.5 kg), supplemental mixture (6.7 kg) and mineral mixture (0.1 kg, all on as-fed basis). Samples of the same diet modified to forage:concentrate ratio of 60:40 were incubated in buffered rumen fluid at 39°C for 24 h with addition of methanol (K+) or enniatin B in concentrations either 10 ?g/ml (E10) or 20 ?g/ml (E20) and compared to control (K-). In vitro organic matter digestibility differed among groups (P&lt;0.05) and was 34.3, 32.8, 36.1 and 31.4 % in K-, K+, E10 and E20, respectively. Results suggested that higher levels of enniatin B in the diet may have inhibitory effect on rumen microbiota and can negatively influence the dry matter digestibility.

Název v anglickém jazyce

The effect of minor mycotoxin enniatin B on in vitro dry matter digestibility

Popis výsledku anglicky

The aim of this study was to determine the effect of minor mycotoxin enniatin B (Sigma-Aldrich, dissolved in methanol) on dry matter digestibility of total mixed ration. The experiments were performed in vitro using a rumen fluid-buffer system. Rumen fluid was taken from dairy cows fed on diet consisted of maize silage (22 kg), lucerne haylage (12 kg), meadow hay (3 kg), feeding straw (1.5 kg), supplemental mixture (6.7 kg) and mineral mixture (0.1 kg, all on as-fed basis). Samples of the same diet modified to forage:concentrate ratio of 60:40 were incubated in buffered rumen fluid at 39°C for 24 h with addition of methanol (K+) or enniatin B in concentrations either 10 ?g/ml (E10) or 20 ?g/ml (E20) and compared to control (K-). In vitro organic matter digestibility differed among groups (P&lt;0.05) and was 34.3, 32.8, 36.1 and 31.4 % in K-, K+, E10 and E20, respectively. Results suggested that higher levels of enniatin B in the diet may have inhibitory effect on rumen microbiota and can negatively influence the dry matter digestibility.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Sborník Animal Physiology, Nutrition and Welfare 2018

ISBN

978-80-7394-715-6

ISSN

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e-ISSN

neuvedeno

Počet stran výsledku

7

Strana od-do

211-217

Název nakladatele

Jihočeská univerzita v Českých Budějovicích

Místo vydání

České Budějovice

Místo konání akce

České Budějovice

Datum konání akce

21. 11. 2018

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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