Toxicity of hydrogen sulfide toward sulfate-reducing bacteria Desulfovibrio piger Vib-7

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F19%3A43877675" target="_blank" >RIV/62157124:16270/19:43877675 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/19:00109107

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s00203-019-01625-z#citeas" target="_blank" >https://link.springer.com/article/10.1007/s00203-019-01625-z#citeas</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00203-019-01625-z" target="_blank" >10.1007/s00203-019-01625-z</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Toxicity of hydrogen sulfide toward sulfate-reducing bacteria Desulfovibrio piger Vib-7

Popis výsledku v původním jazyce

Sulfate-reducing bacteria (SRB) belonging to the intestinal microbiota are the main producers of hydrogen sulfide and their increasing amount due to the accumulation of this compound in the bowel are involved in the initiation and maintenance of inflammatory bowel disease. The purpose of this experiment is to study the relative toxicity of hydrogen sulfide and survival of Desulfovibrio piger Vib-7 through monitoring: sulfate reduction parameters (sulfate consumption, hydrogen sulfide production, lactate consumption and acetate production) and kinetic parameters of these processes. The research is highlighting the survival of intestinal SRB, D. piger Vib-7 under the influence of different hydrogen sulfide concentrations (1-7mM). The highest toxicity of H2S was measured in the presence of concentrations higher than 6mM, where growing was stopped, though metabolic activities were not 100% inhibited. These findings are confirmed by cross correlation and principal component analysis that clearly supported the above mentioned results. The kinetic parameters of bacterial growth and sulfate reduction were inhibited proportionally with increasing H2S concentration. The presence of 5 mM H2S resulted in two times longer lag phase and generation time was eight times longer. Maximum rate of growth and hydrogen production was stopped under 4 mM, emphasizing the H2S toxicity concentrations to be &lt; 4mM, even for sulfide producing bacteria such as Desulfovibrio. The results are confirming H2S concentrations toxicity toward Desulfovibrio, especially the study novelty should be emphasized where it was found that the exact H2S limits (&gt; 4mM) toward this bacterial strain inhabiting humans and animals intestine.

Název v anglickém jazyce

Toxicity of hydrogen sulfide toward sulfate-reducing bacteria Desulfovibrio piger Vib-7

Popis výsledku anglicky

Sulfate-reducing bacteria (SRB) belonging to the intestinal microbiota are the main producers of hydrogen sulfide and their increasing amount due to the accumulation of this compound in the bowel are involved in the initiation and maintenance of inflammatory bowel disease. The purpose of this experiment is to study the relative toxicity of hydrogen sulfide and survival of Desulfovibrio piger Vib-7 through monitoring: sulfate reduction parameters (sulfate consumption, hydrogen sulfide production, lactate consumption and acetate production) and kinetic parameters of these processes. The research is highlighting the survival of intestinal SRB, D. piger Vib-7 under the influence of different hydrogen sulfide concentrations (1-7mM). The highest toxicity of H2S was measured in the presence of concentrations higher than 6mM, where growing was stopped, though metabolic activities were not 100% inhibited. These findings are confirmed by cross correlation and principal component analysis that clearly supported the above mentioned results. The kinetic parameters of bacterial growth and sulfate reduction were inhibited proportionally with increasing H2S concentration. The presence of 5 mM H2S resulted in two times longer lag phase and generation time was eight times longer. Maximum rate of growth and hydrogen production was stopped under 4 mM, emphasizing the H2S toxicity concentrations to be &lt; 4mM, even for sulfide producing bacteria such as Desulfovibrio. The results are confirming H2S concentrations toxicity toward Desulfovibrio, especially the study novelty should be emphasized where it was found that the exact H2S limits (&gt; 4mM) toward this bacterial strain inhabiting humans and animals intestine.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Archives of microbiology

ISSN

0302-8933

e-ISSN

—

Svazek periodika

201

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

389-397

Kód UT WoS článku

000462145000012

EID výsledku v databázi Scopus

2-s2.0-85061004406