Analysis of pH dose-dependent growth of sulfate-reducing bacteria

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F19%3A43877676" target="_blank" >RIV/62157124:16270/19:43877676 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/19:00109053

Výsledek na webu

<a href="https://www.degruyter.com/view/j/med.2019.14.issue-1/med-2019-0010/med-2019-0010.xml" target="_blank" >https://www.degruyter.com/view/j/med.2019.14.issue-1/med-2019-0010/med-2019-0010.xml</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1515/med-2019-0010" target="_blank" >10.1515/med-2019-0010</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of pH dose-dependent growth of sulfate-reducing bacteria

Popis výsledku v původním jazyce

Lower intraluminal colonic pH is an indication for the development of inflammatory bowel disease including active ulcerative colitis. Involvement of intestinal sulfate-reducing bacteria in decreasing bowel pH by the production of H2S and acetate as well as their sensitivity has never been reported before. The study of the relative pH and survival of Desulfovibrio piger Vib-7 by monitoring sulfate reduction parameters was the aim of this work. Monitoring was done through the measurement of bacterial growth (biomass), dissimilatory sulfate reduction parameters: sulfate consumption, lactate oxidation, hydrogen sulfide and acetate production. According to our results, we observed that lower pH (&lt;5) significantly inhibited D. piger Vib-7 growth. This inhibition was also noticed when alkaline media (&gt;9 pH) was used, though the reduction was not at the rate as in media with pH of 4. The research indicates that the growth of D. piger Vib-7 is inhibited at pH of 4 which is not as low as the pH found in people with severely developed inflammatory bowel diseases such as ulcerative colitis. Certainly the interaction (synergistic effect) between both hydrogen sulfide and acetate accumulation can also play an important etiological role in the development of bowel inflammation in humans and animals.

Název v anglickém jazyce

Analysis of pH dose-dependent growth of sulfate-reducing bacteria

Popis výsledku anglicky

Lower intraluminal colonic pH is an indication for the development of inflammatory bowel disease including active ulcerative colitis. Involvement of intestinal sulfate-reducing bacteria in decreasing bowel pH by the production of H2S and acetate as well as their sensitivity has never been reported before. The study of the relative pH and survival of Desulfovibrio piger Vib-7 by monitoring sulfate reduction parameters was the aim of this work. Monitoring was done through the measurement of bacterial growth (biomass), dissimilatory sulfate reduction parameters: sulfate consumption, lactate oxidation, hydrogen sulfide and acetate production. According to our results, we observed that lower pH (&lt;5) significantly inhibited D. piger Vib-7 growth. This inhibition was also noticed when alkaline media (&gt;9 pH) was used, though the reduction was not at the rate as in media with pH of 4. The research indicates that the growth of D. piger Vib-7 is inhibited at pH of 4 which is not as low as the pH found in people with severely developed inflammatory bowel diseases such as ulcerative colitis. Certainly the interaction (synergistic effect) between both hydrogen sulfide and acetate accumulation can also play an important etiological role in the development of bowel inflammation in humans and animals.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Open Medicine

ISSN

2391-5463

e-ISSN

—

Svazek periodika

14

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

9

Strana od-do

66-74

Kód UT WoS článku

000457914100003

EID výsledku v databázi Scopus

2-s2.0-85062870398