Hibernation strategy: functions of bat-derived cell lines at low temperatures

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F21%3A43879608" target="_blank" >RIV/62157124:16270/21:43879608 - isvavai.cz</a>

Výsledek na webu

<a href="https://fvhe.vfu.cz/files/upload/KMVP%202021_Sborn%C3%ADk_1.pdf" target="_blank" >https://fvhe.vfu.cz/files/upload/KMVP%202021_Sborn%C3%ADk_1.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Hibernation strategy: functions of bat-derived cell lines at low temperatures

Popis výsledku v původním jazyce

Heterothermy is bat adaptive strategy of controlled regulation of their body temperature to cope with seasonal climatic extremes with unavailability of insect prey. During hibernation and daily torpor, body temperature significantly decreases. Therefore, it is essential that tissues and cells are adapted on these temperature changes. We observed phagocytic intensity, proliferation and morphology of bat macrophage cell line at different temperatures simulating body temperature during hibernation (8 °C), daily torpor (17.5 °C) and euthermy (37 °C) and survival of cells obtained from different organ under experimental temperature -20 °C in medium without externally added cryoprotectant. Bat cells are adapted for significantly decreased incubation ~ body temperature. Cells maintain viability and functions at low temperatures, but the proliferation rate and intensity of phagocytosis decrease compared to euthermic conditions. Cells at 8 and -20 °C stay round shaped in suspensionvand form a few-number cell clusters. Cells of different tissues differ in their ability to survive extreme decrease and subsequent increase of temperature. The lowest viability in vitro was observed in body core organ cells (liver), contrariwise cells close to the body surface (nerves) show high viability and rapid regrowth under optimal conditions. We conclude, bat cells are adapted to overcome low temperatures using hypometabolism.

Název v anglickém jazyce

Hibernation strategy: functions of bat-derived cell lines at low temperatures

Popis výsledku anglicky

Heterothermy is bat adaptive strategy of controlled regulation of their body temperature to cope with seasonal climatic extremes with unavailability of insect prey. During hibernation and daily torpor, body temperature significantly decreases. Therefore, it is essential that tissues and cells are adapted on these temperature changes. We observed phagocytic intensity, proliferation and morphology of bat macrophage cell line at different temperatures simulating body temperature during hibernation (8 °C), daily torpor (17.5 °C) and euthermy (37 °C) and survival of cells obtained from different organ under experimental temperature -20 °C in medium without externally added cryoprotectant. Bat cells are adapted for significantly decreased incubation ~ body temperature. Cells maintain viability and functions at low temperatures, but the proliferation rate and intensity of phagocytosis decrease compared to euthermic conditions. Cells at 8 and -20 °C stay round shaped in suspensionvand form a few-number cell clusters. Cells of different tissues differ in their ability to survive extreme decrease and subsequent increase of temperature. The lowest viability in vitro was observed in body core organ cells (liver), contrariwise cells close to the body surface (nerves) show high viability and rapid regrowth under optimal conditions. We conclude, bat cells are adapted to overcome low temperatures using hypometabolism.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

XXIlI. Konference mladých vědeckých pracovníků s mezinárodní účastí

ISBN

978-80-7305-851-7

ISSN

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e-ISSN

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Počet stran výsledku

3

Strana od-do

76-78

Název nakladatele

Veterinární univerzita Brno

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

14. 6. 2021

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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