Evaluation of the glutathione concentration in whole blood of dairy Holstein cows
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F21%3A43879719" target="_blank" >RIV/62157124:16270/21:43879719 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.agriculturejournals.cz/web/vetmed.htm?type=article&id=157_2020-VETMED" target="_blank" >https://www.agriculturejournals.cz/web/vetmed.htm?type=article&id=157_2020-VETMED</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.17221/157/2020-VETMED" target="_blank" >10.17221/157/2020-VETMED</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Evaluation of the glutathione concentration in whole blood of dairy Holstein cows
Popis výsledku v původním jazyce
The objective of this study was to evaluate the concentrations of the total ( T-GSH), oxidised (GSSG) and reduced (GSH) glutathione in the blood of dairy cows, assess the relationships of the phase and number of lactation, milk production, body condition score (BCS) and selected biochemical parameters on its concentrations. We analysed 79 samples of whole blood from dairy Holstein cows. The concentration of glutathione was assessed by the spectrophotometric enzymatic method. The whole dataset shows the mean concentration of T-GSH, GSSG and GSH of 803 +/- 22 mu mol/l, 23 +/- 2.5 mu mol/l and 757 +/- 27 mu mol/l, respectively. The GSH/GSSG ratio was 95 +/- 17. The phase of the lactation had a significant impact on the levels of T-GSH and GSH, but the number of lactation and BCS had no effect. Dry cows had higher levels of T-GSH ( 938 +/- 44 mu mol/l) than the fresh (713 +/- 46 mu mol/l) and peak lactation (785 +/- 45 mu mol/l) cows. The fresh cows had significantly lower concentrations of GSH (618 +/- 44 mu mol/l) than the peak lactation ( 719 +/- 46 mu mol/l) and dry cows ( 827 +/- 43 mu mol/l). On the basis of a regression analysis, blood glutathione was affected mainly by the liver function and energy metabolism. Glutathione as the marker of oxidation stress seems to be a promising tool in monitoring the health and welfare of the herd, yet intensive research in this field remains necessary.
Název v anglickém jazyce
Evaluation of the glutathione concentration in whole blood of dairy Holstein cows
Popis výsledku anglicky
The objective of this study was to evaluate the concentrations of the total ( T-GSH), oxidised (GSSG) and reduced (GSH) glutathione in the blood of dairy cows, assess the relationships of the phase and number of lactation, milk production, body condition score (BCS) and selected biochemical parameters on its concentrations. We analysed 79 samples of whole blood from dairy Holstein cows. The concentration of glutathione was assessed by the spectrophotometric enzymatic method. The whole dataset shows the mean concentration of T-GSH, GSSG and GSH of 803 +/- 22 mu mol/l, 23 +/- 2.5 mu mol/l and 757 +/- 27 mu mol/l, respectively. The GSH/GSSG ratio was 95 +/- 17. The phase of the lactation had a significant impact on the levels of T-GSH and GSH, but the number of lactation and BCS had no effect. Dry cows had higher levels of T-GSH ( 938 +/- 44 mu mol/l) than the fresh (713 +/- 46 mu mol/l) and peak lactation (785 +/- 45 mu mol/l) cows. The fresh cows had significantly lower concentrations of GSH (618 +/- 44 mu mol/l) than the peak lactation ( 719 +/- 46 mu mol/l) and dry cows ( 827 +/- 43 mu mol/l). On the basis of a regression analysis, blood glutathione was affected mainly by the liver function and energy metabolism. Glutathione as the marker of oxidation stress seems to be a promising tool in monitoring the health and welfare of the herd, yet intensive research in this field remains necessary.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
40301 - Veterinary science
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Veterinární medicína
ISSN
0375-8427
e-ISSN
—
Svazek periodika
66
Číslo periodika v rámci svazku
5
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
10
Strana od-do
179-188
Kód UT WoS článku
000647289900001
EID výsledku v databázi Scopus
2-s2.0-85107475060