Capillary Isotachophoresis Determination of Trace Oxidized Glutathione in Blood

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F18%3A43876897" target="_blank" >RIV/62157124:16270/18:43876897 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1515/hjic-2018-0004" target="_blank" >http://dx.doi.org/10.1515/hjic-2018-0004</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1515/hjic-2018-0004" target="_blank" >10.1515/hjic-2018-0004</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Capillary Isotachophoresis Determination of Trace Oxidized Glutathione in Blood

Popis výsledku v původním jazyce

A capillary isotachophoresis (CITP) method performed in a column-coupling apparatus has been developed for the simultaneous determination of glutathione (GSH) and glutathione disulfide (GSSG) concentrations in blood samples. The determination of GSSG and GSH concentrations in biological samples is important because of their roles in oxidative stress. Different concentrations of a leading ion in the coupled columns (concentration cascade) and a large volume (37 mu l) of the injected sample facilitated a GSSG concentration of between 2 and 25 mu mol/l. A reaction between iodoacetate and GSH under alkaline conditions was used to prepare the sample in order to avoid oxidation of GSH to GSSG. This step eliminated the main source of systematic errors with regard to the determination of the GSSG concentration. A linear relationship (R-2 = 0.9969) between the zone length of S-(carboxymethyl) glutathione (the product of the reaction between GSH and iodoacetate) and the concentration of GSH (40-120 mu mol/l) was obtained. The method was applied to the analysis of bovine blood samples that had been diluted by a factor of ten with satisfactory results.

Název v anglickém jazyce

Capillary Isotachophoresis Determination of Trace Oxidized Glutathione in Blood

Popis výsledku anglicky

A capillary isotachophoresis (CITP) method performed in a column-coupling apparatus has been developed for the simultaneous determination of glutathione (GSH) and glutathione disulfide (GSSG) concentrations in blood samples. The determination of GSSG and GSH concentrations in biological samples is important because of their roles in oxidative stress. Different concentrations of a leading ion in the coupled columns (concentration cascade) and a large volume (37 mu l) of the injected sample facilitated a GSSG concentration of between 2 and 25 mu mol/l. A reaction between iodoacetate and GSH under alkaline conditions was used to prepare the sample in order to avoid oxidation of GSH to GSSG. This step eliminated the main source of systematic errors with regard to the determination of the GSSG concentration. A linear relationship (R-2 = 0.9969) between the zone length of S-(carboxymethyl) glutathione (the product of the reaction between GSH and iodoacetate) and the concentration of GSH (40-120 mu mol/l) was obtained. The method was applied to the analysis of bovine blood samples that had been diluted by a factor of ten with satisfactory results.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Hungarian journal of industry and chemistry

ISSN

0133-0276

e-ISSN

—

Svazek periodika

46

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

5

Strana od-do

13-17

Kód UT WoS článku

000440079700004

EID výsledku v databázi Scopus

—