Determination of glutathione and glutathione disulfide in human whole blood using HPLC with coulometric detection: a comparison with fluorescence detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F11%3A10105719" target="_blank" >RIV/00216208:11150/11:10105719 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216275:25310/11:39892024

Výsledek na webu

<a href="http://cccc.uochb.cas.cz/76/4/0277/" target="_blank" >http://cccc.uochb.cas.cz/76/4/0277/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1135/cccc2010038" target="_blank" >10.1135/cccc2010038</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of glutathione and glutathione disulfide in human whole blood using HPLC with coulometric detection: a comparison with fluorescence detection

Popis výsledku v původním jazyce

We describe a relatively simple method for the determination of glutathione (GSH) and glutathione disulfide (GSSG) in human whole blood. We have used an HPLC with coulometric electrochemical detection for the s?taneous measurement of GSH and GSSG. Diluted and filtered trichloroacetic acid extracts were injected directly into the HPLC system and were eluted isocratically on a Polaris 5u C18-A, 250 x 4.6 mm analytical column. Glutathione in samples extracted with trichloroacetic acid and diluted with 1.0mmol/l hydrochloric acid was stable at 4 oC for at least 8 h. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries from spiked whole blood samples were at intervals 91.6-97.6% for GSH and 85.0-104.4% for GSSG. The linear range is 5.0-2000.0 ?mol/l, with a detection limit of 2.1 ?mol/l (signal-to-noise ratio = 3) for GSH and 2.0-250.0 ?mol/l, with a detection limit of 0.9 ?mol/l for GSS

Název v anglickém jazyce

Determination of glutathione and glutathione disulfide in human whole blood using HPLC with coulometric detection: a comparison with fluorescence detection

Popis výsledku anglicky

We describe a relatively simple method for the determination of glutathione (GSH) and glutathione disulfide (GSSG) in human whole blood. We have used an HPLC with coulometric electrochemical detection for the s?taneous measurement of GSH and GSSG. Diluted and filtered trichloroacetic acid extracts were injected directly into the HPLC system and were eluted isocratically on a Polaris 5u C18-A, 250 x 4.6 mm analytical column. Glutathione in samples extracted with trichloroacetic acid and diluted with 1.0mmol/l hydrochloric acid was stable at 4 oC for at least 8 h. The analytical performance of this method is satisfactory: the intra-assay and inter-assay coefficients of variation were below 10%. Quantitative recoveries from spiked whole blood samples were at intervals 91.6-97.6% for GSH and 85.0-104.4% for GSSG. The linear range is 5.0-2000.0 ?mol/l, with a detection limit of 2.1 ?mol/l (signal-to-noise ratio = 3) for GSH and 2.0-250.0 ?mol/l, with a detection limit of 0.9 ?mol/l for GSS

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Collection of Czechoslovak Chemical Communications

ISSN

0010-0765

e-ISSN

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Svazek periodika

76

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

18

Strana od-do

277-294

Kód UT WoS článku

000289355200005

EID výsledku v databázi Scopus

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