Cryoprotective potential of urea in bat cells in vitro

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16270%2F24%3A43881501" target="_blank" >RIV/62157124:16270/24:43881501 - isvavai.cz</a>

Výsledek na webu

<a href="https://actavet.vfu.cz/93/3/0331/" target="_blank" >https://actavet.vfu.cz/93/3/0331/</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2754/avb202493030331" target="_blank" >10.2754/avb202493030331</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cryoprotective potential of urea in bat cells in vitro

Popis výsledku v původním jazyce

Hibernating bats potentially risk cold-induced cell damage. However, the cells of hibernating bats have previously been shown to be able to cope with sub-zero temperatures, either alone or due to glucose cryoprotection. The aim of our study was to determine the survival rate of bat-derived cells in vitro after 24 h at -20 degrees C in a medium supplemented with urea at concentrations ranging from 0 to 320 mM or with a urea-glucose combination. Urea showed limited cryoprotective potential, with cell survival rates not exceeding 3% at urea concentrations of 20 to 40 mM, which corresponds to reported levels for blood urea in bats. Higher urea concentrations tended to be toxic rather than protective. Importantly, urea appeared to relieve delayed-onset cell death after cell rewarming, and also improved the cryoprotective effect of glucose up to three-fold. Thus, supplementation of cell cultures with a cryoprotectant mix containing urea could increase cell survival rates.

Název v anglickém jazyce

Cryoprotective potential of urea in bat cells in vitro

Popis výsledku anglicky

Hibernating bats potentially risk cold-induced cell damage. However, the cells of hibernating bats have previously been shown to be able to cope with sub-zero temperatures, either alone or due to glucose cryoprotection. The aim of our study was to determine the survival rate of bat-derived cells in vitro after 24 h at -20 degrees C in a medium supplemented with urea at concentrations ranging from 0 to 320 mM or with a urea-glucose combination. Urea showed limited cryoprotective potential, with cell survival rates not exceeding 3% at urea concentrations of 20 to 40 mM, which corresponds to reported levels for blood urea in bats. Higher urea concentrations tended to be toxic rather than protective. Importantly, urea appeared to relieve delayed-onset cell death after cell rewarming, and also improved the cryoprotective effect of glucose up to three-fold. Thus, supplementation of cell cultures with a cryoprotectant mix containing urea could increase cell survival rates.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

40301 - Veterinary science

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta veterinaria Brno

ISSN

0001-7213

e-ISSN

1801-7576

Svazek periodika

93

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

331-337

Kód UT WoS článku

001338130400010

EID výsledku v databázi Scopus

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