Genomics of multi-resistant Escherichia coli circulating in a colony of gulls: Dynamics of colonizing strains displays importance of longitudinal wildlife studies

Kód výsledku v IS VaVaI

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RIV/62157124:16810/24:43881595

Výsledek na webu

<a href="https://fvhe.vetuni.cz/files/upload/733/KMVP%202024_Sbornik.pdf" target="_blank" >https://fvhe.vetuni.cz/files/upload/733/KMVP%202024_Sbornik.pdf</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Genomics of multi-resistant Escherichia coli circulating in a colony of gulls: Dynamics of colonizing strains displays importance of longitudinal wildlife studies

Popis výsledku v původním jazyce

Migrating birds play an important role in dispersing resistant bacteria all over the globe. They are in contact with many environments including the anthropogenically influenced ones such as landfills where they can obtain resistant strains while feeding. These strains can then exchange plasmids which have a crucial part in the spread of resistance between bacteria. We analysed the dynamics of cephalosporin-resistant Escherichia coli and diffusion of plasmids encoding cephalosporinases in the population of Caspian gulls nesting at the water reservoir Nové Mlýny. During our experiment, five non-flying gull nestlings were placed together into an aviary for 12 weeks. Every two weeks, cloacal swabs were obtained and selective cultivation on media with cefotaxime was performed. Representative resistant isolates were subjected to whole genome sequencing. Data showed changes in the prevalence of particular strains including their transmission between individual birds. At the beginning of the experiment, all the gulls except for one were colonized by a strain of E. coli with an F-type plasmid carrying blaCMY-2 gene. During the second sampling, all the gulls already carried an E. coli with different plasmid IncI1 with blaCTX-M gene, which was originally present only in one bird. At the end of the experiment, the colonization of the birds started to recede but did not disappear completely. This displays that resistant bacteria can persist in birds for a sufficiently long period to be further disseminated.

Název v anglickém jazyce

Genomics of multi-resistant Escherichia coli circulating in a colony of gulls: Dynamics of colonizing strains displays importance of longitudinal wildlife studies

Popis výsledku anglicky

Migrating birds play an important role in dispersing resistant bacteria all over the globe. They are in contact with many environments including the anthropogenically influenced ones such as landfills where they can obtain resistant strains while feeding. These strains can then exchange plasmids which have a crucial part in the spread of resistance between bacteria. We analysed the dynamics of cephalosporin-resistant Escherichia coli and diffusion of plasmids encoding cephalosporinases in the population of Caspian gulls nesting at the water reservoir Nové Mlýny. During our experiment, five non-flying gull nestlings were placed together into an aviary for 12 weeks. Every two weeks, cloacal swabs were obtained and selective cultivation on media with cefotaxime was performed. Representative resistant isolates were subjected to whole genome sequencing. Data showed changes in the prevalence of particular strains including their transmission between individual birds. At the beginning of the experiment, all the gulls except for one were colonized by a strain of E. coli with an F-type plasmid carrying blaCMY-2 gene. During the second sampling, all the gulls already carried an E. coli with different plasmid IncI1 with blaCTX-M gene, which was originally present only in one bird. At the end of the experiment, the colonization of the birds started to recede but did not disappear completely. This displays that resistant bacteria can persist in birds for a sufficiently long period to be further disseminated.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10606 - Microbiology

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

XXVI. konference mladých vědeckých pracovníků s mezinárodní účastí: Sborník příspěvků

ISBN

978-80-7305-957-6

ISSN

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e-ISSN

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Počet stran výsledku

3

Strana od-do

65-67

Název nakladatele

Veterinární univerzita Brno

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

5. 6. 2024

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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