Characterisation of Colistin -Resistant Enterobacterales and Acinetobacter Strains Carrying mcr Genes from Asian Aquaculture Products

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00027162%3A_____%2F21%3AN0000102" target="_blank" >RIV/00027162:_____/21:N0000102 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14310/21:00121998

Výsledek na webu

<a href="https://www.mdpi.com/2079-6382/10/7/838" target="_blank" >https://www.mdpi.com/2079-6382/10/7/838</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/antibiotics10070838" target="_blank" >10.3390/antibiotics10070838</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Characterisation of Colistin -Resistant Enterobacterales and Acinetobacter Strains Carrying mcr Genes from Asian Aquaculture Products

Popis výsledku v původním jazyce

Aquaculture systems are widely recognized as hotspots for horizontal gene transfer and the need for screening for bacteria carrying antimicrobial resistance genes in aquaculture systems is becoming more important. In this study, we characterised seventeen bacterial strains (Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, and A. nosocomialis) resistant to colistin originating from retailed aquaculture products imported from Vietnam to the Czech Republic. The mcr-1.1 gene was found located on plasmid types IncHI2, IncI2, IncX4 as well as on rarely described plasmid types IncFIB-FIC and IncFIB(K), phage-like plasmid p0111, or in the chromosome of E. coli. One E. coli strain carried the mcr-3.5 gene on IncFII(pCoo) plasmid in addition to the mcr-1.1 gene located on IncHI2 plasmid. K. pneumoniae was found to carry the mcr-1.1 and mcr-8.2 genes on IncFIA(HI1) plasmid. The mcr-4.3 gene was found on similar untypeable plasmids of A. baumannii and A. nosocomialis strains pointing to possible interspecies transfer of plasmids carrying the mcr-4 gene. Our results highlight that aquaculture products of Asian origin can represent an important source of variable plasmids carrying mcr genes. The results showed an involvement of phages in the incorporation of the mcr-1 gene into plasmids or the chromosome in E. coli strains from aquaculture. The detection of E. coli with the mcr-1 gene in the chromosome points out the risks associated with the stabilization of the mcr genes in bacterial chromosome.

Název v anglickém jazyce

Characterisation of Colistin -Resistant Enterobacterales and Acinetobacter Strains Carrying mcr Genes from Asian Aquaculture Products

Popis výsledku anglicky

Aquaculture systems are widely recognized as hotspots for horizontal gene transfer and the need for screening for bacteria carrying antimicrobial resistance genes in aquaculture systems is becoming more important. In this study, we characterised seventeen bacterial strains (Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, and A. nosocomialis) resistant to colistin originating from retailed aquaculture products imported from Vietnam to the Czech Republic. The mcr-1.1 gene was found located on plasmid types IncHI2, IncI2, IncX4 as well as on rarely described plasmid types IncFIB-FIC and IncFIB(K), phage-like plasmid p0111, or in the chromosome of E. coli. One E. coli strain carried the mcr-3.5 gene on IncFII(pCoo) plasmid in addition to the mcr-1.1 gene located on IncHI2 plasmid. K. pneumoniae was found to carry the mcr-1.1 and mcr-8.2 genes on IncFIA(HI1) plasmid. The mcr-4.3 gene was found on similar untypeable plasmids of A. baumannii and A. nosocomialis strains pointing to possible interspecies transfer of plasmids carrying the mcr-4 gene. Our results highlight that aquaculture products of Asian origin can represent an important source of variable plasmids carrying mcr genes. The results showed an involvement of phages in the incorporation of the mcr-1 gene into plasmids or the chromosome in E. coli strains from aquaculture. The detection of E. coli with the mcr-1 gene in the chromosome points out the risks associated with the stabilization of the mcr genes in bacterial chromosome.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/NV18-09-00254" target="_blank" >NV18-09-00254: Sentinelová studie výskytu na plazmid vázané (přenositelné) rezistence ke kolistinu u Enterobacteriaceae v České republice</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Antibiotics

ISSN

2079-6382

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

16

Strana od-do

—

Kód UT WoS článku

000675944600001

EID výsledku v databázi Scopus

2-s2.0-85110884366