Fast separation and determination of free myo-inositol by hydrophilic liquid chromatography

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F14%3A43872716" target="_blank" >RIV/62157124:16370/14:43872716 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.carres.2014.03.010" target="_blank" >http://dx.doi.org/10.1016/j.carres.2014.03.010</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.carres.2014.03.010" target="_blank" >10.1016/j.carres.2014.03.010</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Fast separation and determination of free myo-inositol by hydrophilic liquid chromatography

Popis výsledku v původním jazyce

A fast liquid chromatography method for separation and determination of myo-inositol is reported. Determination of the biologically important isomer of inositols, myo-inositol, was optimized to avoid overlapping to possible interferents according to European Pharmacopoeia (glycerol, D-mannitol) and saccharose. The method in HILIC mode is extremely selective to other carbohydrates which allows to separate myo-inositol from allo-and D-chiro-inositol with resolution 12.3 and 5.2, resp. and this way it enables to separate myo-inostiol from contingent carbohydrates present in a sample matrix. Retention time of myo-inositol was 12 min at 10 degrees C, though higher temperatures (25 degrees C or 40 degrees C) or higher water content in the mobile phase couldspeed up the separation and determination to four minutes. LOD of the method was 9 mg/L at 10 degrees C, and 5 mg/L at 25 degrees C, resp.

Název v anglickém jazyce

Fast separation and determination of free myo-inositol by hydrophilic liquid chromatography

Popis výsledku anglicky

A fast liquid chromatography method for separation and determination of myo-inositol is reported. Determination of the biologically important isomer of inositols, myo-inositol, was optimized to avoid overlapping to possible interferents according to European Pharmacopoeia (glycerol, D-mannitol) and saccharose. The method in HILIC mode is extremely selective to other carbohydrates which allows to separate myo-inositol from allo-and D-chiro-inositol with resolution 12.3 and 5.2, resp. and this way it enables to separate myo-inostiol from contingent carbohydrates present in a sample matrix. Retention time of myo-inositol was 12 min at 10 degrees C, though higher temperatures (25 degrees C or 40 degrees C) or higher water content in the mobile phase couldspeed up the separation and determination to four minutes. LOD of the method was 9 mg/L at 10 degrees C, and 5 mg/L at 25 degrees C, resp.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FR - Farmakologie a lékárnická chemie

OECD FORD obor

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Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Carbohydrate Research

ISSN

0008-6215

e-ISSN

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Svazek periodika

391

Číslo periodika v rámci svazku

JUN 2014

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

55-60

Kód UT WoS článku

000335902600011

EID výsledku v databázi Scopus

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