Physiology and methodology of chromium toxicity using alga Scenedesmus quadricauda as model object

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16370%2F15%3A43873465" target="_blank" >RIV/62157124:16370/15:43873465 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26220/14:PU109573 RIV/00159816:_____/15:00061108 RIV/62156489:43210/15:43906311

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.chemosphere.2014.05.074" target="_blank" >http://dx.doi.org/10.1016/j.chemosphere.2014.05.074</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chemosphere.2014.05.074" target="_blank" >10.1016/j.chemosphere.2014.05.074</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Physiology and methodology of chromium toxicity using alga Scenedesmus quadricauda as model object

Popis výsledku v původním jazyce

Physiological responses of Scenedesmus quadricauda to Cr(VI) and Cr(III) excess were studied in buffer with circumneutral pH (6.5). Total Cr content was similar in low (1 mu M of both oxidation states) but higher in 10 WO Cr(VI) treatment and high accumulation potential was detected (80-82% and 41-65% in 1 and 10 mu M treatments, respectively). Specific fluorescence indicator (6-((anthracen-9-yl)methyleneamino)-2H-chromen-2-one) confirmed partial reduction of Cr(VI) to Cr(III) under exposure conditions. Viability and chlorophyll autofluorescence were more depleted by Cr(VI) while Cr(III) stimulated increase in ROS and lipid peroxidation. Antioxidative enzyme activities showed significantly higher values in 10 mu M treatments of both Cr oxidation states. Depletion of mitochondrial proteins was not reflected in alteration of total soluble proteins indicating sensitivity of this organelle to Cr and TTC test showed no clear oxidation state-related effect. In this view, "Cr(VI) is not more toxic than Cr(III)" at least for some parameters. Subsequent study with the application of 10 mu M Cr(VI) confirmed that HEPES buffer is more suitable exposure solution for toxicological studied than water or inorganic salts (higher chlorophyll autofluorescence was observed) and pH 6.5 is more suitable than low or high pH (4.5 or 8.5) in terms of Cr uptake. Another known Cr(III) fluorescence indicator (naphthalimide rhodamine) also confirmed partial reduction of Cr(VI) to Cr(III) at acidic pH but only traces were seen at alkaline pH.

Název v anglickém jazyce

Physiology and methodology of chromium toxicity using alga Scenedesmus quadricauda as model object

Popis výsledku anglicky

Physiological responses of Scenedesmus quadricauda to Cr(VI) and Cr(III) excess were studied in buffer with circumneutral pH (6.5). Total Cr content was similar in low (1 mu M of both oxidation states) but higher in 10 WO Cr(VI) treatment and high accumulation potential was detected (80-82% and 41-65% in 1 and 10 mu M treatments, respectively). Specific fluorescence indicator (6-((anthracen-9-yl)methyleneamino)-2H-chromen-2-one) confirmed partial reduction of Cr(VI) to Cr(III) under exposure conditions. Viability and chlorophyll autofluorescence were more depleted by Cr(VI) while Cr(III) stimulated increase in ROS and lipid peroxidation. Antioxidative enzyme activities showed significantly higher values in 10 mu M treatments of both Cr oxidation states. Depletion of mitochondrial proteins was not reflected in alteration of total soluble proteins indicating sensitivity of this organelle to Cr and TTC test showed no clear oxidation state-related effect. In this view, "Cr(VI) is not more toxic than Cr(III)" at least for some parameters. Subsequent study with the application of 10 mu M Cr(VI) confirmed that HEPES buffer is more suitable exposure solution for toxicological studied than water or inorganic salts (higher chlorophyll autofluorescence was observed) and pH 6.5 is more suitable than low or high pH (4.5 or 8.5) in terms of Cr uptake. Another known Cr(III) fluorescence indicator (naphthalimide rhodamine) also confirmed partial reduction of Cr(VI) to Cr(III) at acidic pH but only traces were seen at alkaline pH.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemosphere

ISSN

0045-6535

e-ISSN

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Svazek periodika

120

Číslo periodika v rámci svazku

feb

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

23-30

Kód UT WoS článku

000348003200004

EID výsledku v databázi Scopus

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