Deproteinization is necessary for the accurate determination of ammonia levels by glutamate dehydrogenase assay in blood plasma from subjects with liver injury

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18450%2F17%3A50013791" target="_blank" >RIV/62690094:18450/17:50013791 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11150/17:10365014

Výsledek na webu

<a href="http://dx.doi.org/10.1093/labmed/lmx053" target="_blank" >http://dx.doi.org/10.1093/labmed/lmx053</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/labmed/lmx053" target="_blank" >10.1093/labmed/lmx053</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Deproteinization is necessary for the accurate determination of ammonia levels by glutamate dehydrogenase assay in blood plasma from subjects with liver injury

Popis výsledku v původním jazyce

Objective: To determine the effect of presence of high concentrations of nicotinamide adenine dinucleotide (NADH)– and nicotinamide adenine dinucleotide phosphate (NADPH)–consuming enzymes on the accuracy of glutamate dehydrogenase (GLDH) assay for ammonia. Methods: We measured ammonia concentrations using GLDH and NADH or NADPH in blood-plasma specimens and specimens deproteinized by sulfosalicylic acid from CCl4-treated or control rats. The nonspecific oxidation of NADH and NADPH was measured in mixtures without GLDH. Results: We observed a gradual decrease (~0.5%) in absorbance in the plasma of controls after the addition of NADH but not after adding NADPH. The decrease in absorbance in plasma of CCl4-treated animals was 13.2% and 5.2% after the addition of NADH and NADPH, respectively. The decrease in absorbance was not detected in deproteinized specimens. The values of ammonia concentration were higher in the plasma specimens compared with the deproteinized ones. Conclusion: Deproteinization is necessary for accurate measurement of ammonia using GLDH assay in the blood plasma of subjects with liver injury.

Název v anglickém jazyce

Deproteinization is necessary for the accurate determination of ammonia levels by glutamate dehydrogenase assay in blood plasma from subjects with liver injury

Popis výsledku anglicky

Objective: To determine the effect of presence of high concentrations of nicotinamide adenine dinucleotide (NADH)– and nicotinamide adenine dinucleotide phosphate (NADPH)–consuming enzymes on the accuracy of glutamate dehydrogenase (GLDH) assay for ammonia. Methods: We measured ammonia concentrations using GLDH and NADH or NADPH in blood-plasma specimens and specimens deproteinized by sulfosalicylic acid from CCl4-treated or control rats. The nonspecific oxidation of NADH and NADPH was measured in mixtures without GLDH. Results: We observed a gradual decrease (~0.5%) in absorbance in the plasma of controls after the addition of NADH but not after adding NADPH. The decrease in absorbance in plasma of CCl4-treated animals was 13.2% and 5.2% after the addition of NADH and NADPH, respectively. The decrease in absorbance was not detected in deproteinized specimens. The values of ammonia concentration were higher in the plasma specimens compared with the deproteinized ones. Conclusion: Deproteinization is necessary for accurate measurement of ammonia using GLDH assay in the blood plasma of subjects with liver injury.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

30105 - Physiology (including cytology)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Laboratory medicine

ISSN

0007-5027

e-ISSN

—

Svazek periodika

48

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

339-345

Kód UT WoS článku

000424018900009

EID výsledku v databázi Scopus

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