Leptospira interrogans outer membrane protein-based nanohybrid sensor for the diagnosis of leptospirosis
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18450%2F21%3A50017989" target="_blank" >RIV/62690094:18450/21:50017989 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.mdpi.com/1424-8220/21/7/2552" target="_blank" >https://www.mdpi.com/1424-8220/21/7/2552</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/s21072552" target="_blank" >10.3390/s21072552</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Leptospira interrogans outer membrane protein-based nanohybrid sensor for the diagnosis of leptospirosis
Popis výsledku v původním jazyce
Leptospirosis is an underestimated tropical disease caused by the pathogenic Leptospira species and responsible for several serious health problems. Here, we aimed to develop an ultrasen-sitive DNA biosensor for the rapid and on-site detection of the Loa22 gene of Leptospira interrogans using a gold nanoparticle–carbon nanofiber composite (AuN/CNF)-based screen-printed electrode. Cyclic voltammetry and electrochemical impedance were performed for electrochemical analysis. The sensitivity of the sensor was 5431.74 µA/cm2 /ng with a LOD (detection limit) of 0.0077 ng/µL using cyclic voltammetry. The developed DNA biosensor was found highly specific to the Loa22 gene of L. interrogans, with a storage stability at 4◦ C for 180 days and a 6% loss of the initial response. This DNA-based sensor only takes 30 min for rapid detection of the pathogen, with a higher specificity and sensitivity. The promising results obtained suggest the application of the developed sensor as a point of care device for the diagnosis of leptospirosis. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Název v anglickém jazyce
Leptospira interrogans outer membrane protein-based nanohybrid sensor for the diagnosis of leptospirosis
Popis výsledku anglicky
Leptospirosis is an underestimated tropical disease caused by the pathogenic Leptospira species and responsible for several serious health problems. Here, we aimed to develop an ultrasen-sitive DNA biosensor for the rapid and on-site detection of the Loa22 gene of Leptospira interrogans using a gold nanoparticle–carbon nanofiber composite (AuN/CNF)-based screen-printed electrode. Cyclic voltammetry and electrochemical impedance were performed for electrochemical analysis. The sensitivity of the sensor was 5431.74 µA/cm2 /ng with a LOD (detection limit) of 0.0077 ng/µL using cyclic voltammetry. The developed DNA biosensor was found highly specific to the Loa22 gene of L. interrogans, with a storage stability at 4◦ C for 180 days and a 6% loss of the initial response. This DNA-based sensor only takes 30 min for rapid detection of the pathogen, with a higher specificity and sensitivity. The promising results obtained suggest the application of the developed sensor as a point of care device for the diagnosis of leptospirosis. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Sensors
ISSN
1424-8220
e-ISSN
—
Svazek periodika
21
Číslo periodika v rámci svazku
7
Stát vydavatele periodika
CH - Švýcarská konfederace
Počet stran výsledku
10
Strana od-do
"Article number 2552"
Kód UT WoS článku
000638865700001
EID výsledku v databázi Scopus
2-s2.0-85103570606