Identification of 17 ‘Candidatus Phytoplasma pyri’ genotypes based on the diversity of the imp gene sequence

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F18%3A50014387" target="_blank" >RIV/62690094:18470/18:50014387 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43510/18:43912338

Výsledek na webu

<a href="http://dx.doi.org/10.1111/ppa.12805" target="_blank" >http://dx.doi.org/10.1111/ppa.12805</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/ppa.12805" target="_blank" >10.1111/ppa.12805</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification of 17 ‘Candidatus Phytoplasma pyri’ genotypes based on the diversity of the imp gene sequence

Popis výsledku v původním jazyce

Phytoplasmas of the apple proliferation group cause diseases in various fruit trees, mainly apples, pears and apricots. Immunodominant membrane protein, or ‘Imp’, shows high inter- and intraspecies gene variability, and is thus frequently used for population studies in these organisms. In this investigation, a survey was carried out of ‘Candidatus Phytoplasma pyri’ imp gene diversity in the Czech Republic. In total, 12 imp genotypes that could be clustered into nine groups were identified. Using all publicly available data, 17 distinct imp genotypes (labelled A–K) were defined. Interestingly, genotypes C, H and I were more related to ‘Ca. Phytoplasma mali’ than to other ‘Ca. Phytoplasma pyri’ imp genotypes, indicating that some strains may represent a transitional type between the two species. As Imp probably plays an important role in host–pathogen interactions, description of its diversity in ‘Ca. Phytoplasma pyri’ may help in understanding the epidemiology, symptomatology, and other aspects of pear decline disease. A simple RFLP PCR system is presented for rapid and cost-effective discrimination of ‘Ca. Phytoplasma pyri’ imp genotypes.

Název v anglickém jazyce

Identification of 17 ‘Candidatus Phytoplasma pyri’ genotypes based on the diversity of the imp gene sequence

Popis výsledku anglicky

Phytoplasmas of the apple proliferation group cause diseases in various fruit trees, mainly apples, pears and apricots. Immunodominant membrane protein, or ‘Imp’, shows high inter- and intraspecies gene variability, and is thus frequently used for population studies in these organisms. In this investigation, a survey was carried out of ‘Candidatus Phytoplasma pyri’ imp gene diversity in the Czech Republic. In total, 12 imp genotypes that could be clustered into nine groups were identified. Using all publicly available data, 17 distinct imp genotypes (labelled A–K) were defined. Interestingly, genotypes C, H and I were more related to ‘Ca. Phytoplasma mali’ than to other ‘Ca. Phytoplasma pyri’ imp genotypes, indicating that some strains may represent a transitional type between the two species. As Imp probably plays an important role in host–pathogen interactions, description of its diversity in ‘Ca. Phytoplasma pyri’ may help in understanding the epidemiology, symptomatology, and other aspects of pear decline disease. A simple RFLP PCR system is presented for rapid and cost-effective discrimination of ‘Ca. Phytoplasma pyri’ imp genotypes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

<a href="/cs/project/QJ1510352" target="_blank" >QJ1510352: Hodnocení faktorů ovlivňujících škodlivost fytoplazem napadajících ovocné dřeviny a ověřování účinných prostředků jejich eliminace</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant pathology

ISSN

0032-0862

e-ISSN

—

Svazek periodika

67

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

971-977

Kód UT WoS článku

000429578000021

EID výsledku v databázi Scopus

2-s2.0-85041115261