Inhibition of cholinesterases following percutaneous intoxication with V agents in rats

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F19%3A50015587" target="_blank" >RIV/62690094:18470/19:50015587 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60162694:G44__/21:00557157

Výsledek na webu

<a href="https://www.tandfonline.com/doi/abs/10.1080/15569543.2018.1564773?journalCode=itxr20" target="_blank" >https://www.tandfonline.com/doi/abs/10.1080/15569543.2018.1564773?journalCode=itxr20</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/15569543.2018.1564773" target="_blank" >10.1080/15569543.2018.1564773</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Inhibition of cholinesterases following percutaneous intoxication with V agents in rats

Popis výsledku v původním jazyce

Female Wistar rats were percutaneously (p.c.) intoxicated (1xLD(50)) with VX and its two derivatives differing in their substitution on nitrogen (diethyl- and dibutyl- derivatives). Blood cholinesterase activity was continuously monitored; 100 min after the intoxication (or after death), acetylcholinesterase (AChE) activity was determined in diaphragm and brain parts (pontomedullar area - PM, frontal cortex - FC, and basal ganglia - BG). Blood ChE activity remains unchanged at very short interval (5 min) after VX administration; this interval was prolonged for diethyl- and dibutyl derivatives. AChE activity was decreased to 20-30% of control values in diaphragm, then in FC (60-70%) and PM (54-74%). AChE activity in BG was relatively resistant (cca 80%). When the AChE activity was compared for all three agents in relationship to survival (11 animals) or death (7 animals), significant differences between the activities in survived (32%) and died (13%) rats were demonstrated in diaphragm but not in the blood. This tendency (higher AChE activity in survived animals) was also observed in PM and FC, however, not statistically significant. It is concluded that both parts of the molecule - substitution on nitrogen atom and the rest of agent molecule (phosphorus head) probably influence penetration through the skin and simultaneously AChE inhibition. As hypothesis, AChE activity in diaphragm could be important for survival or death in case of acute p.c. intoxication with these types of V agents.

Název v anglickém jazyce

Inhibition of cholinesterases following percutaneous intoxication with V agents in rats

Popis výsledku anglicky

Female Wistar rats were percutaneously (p.c.) intoxicated (1xLD(50)) with VX and its two derivatives differing in their substitution on nitrogen (diethyl- and dibutyl- derivatives). Blood cholinesterase activity was continuously monitored; 100 min after the intoxication (or after death), acetylcholinesterase (AChE) activity was determined in diaphragm and brain parts (pontomedullar area - PM, frontal cortex - FC, and basal ganglia - BG). Blood ChE activity remains unchanged at very short interval (5 min) after VX administration; this interval was prolonged for diethyl- and dibutyl derivatives. AChE activity was decreased to 20-30% of control values in diaphragm, then in FC (60-70%) and PM (54-74%). AChE activity in BG was relatively resistant (cca 80%). When the AChE activity was compared for all three agents in relationship to survival (11 animals) or death (7 animals), significant differences between the activities in survived (32%) and died (13%) rats were demonstrated in diaphragm but not in the blood. This tendency (higher AChE activity in survived animals) was also observed in PM and FC, however, not statistically significant. It is concluded that both parts of the molecule - substitution on nitrogen atom and the rest of agent molecule (phosphorus head) probably influence penetration through the skin and simultaneously AChE inhibition. As hypothesis, AChE activity in diaphragm could be important for survival or death in case of acute p.c. intoxication with these types of V agents.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30108 - Toxicology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

TOXIN REVIEWS

ISSN

1556-9543

e-ISSN

—

Svazek periodika

Neuveden

Číslo periodika v rámci svazku

April

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

1-6

Kód UT WoS článku

000470517100001

EID výsledku v databázi Scopus

2-s2.0-85065411147