Towards water-soluble [60]fullerenes for the delivery of siRNA in a prostate cancer model

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F21%3A50018307" target="_blank" >RIV/62690094:18470/21:50018307 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.nature.com/articles/s41598-021-89943-5" target="_blank" >https://www.nature.com/articles/s41598-021-89943-5</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-021-89943-5" target="_blank" >10.1038/s41598-021-89943-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Towards water-soluble [60]fullerenes for the delivery of siRNA in a prostate cancer model

Popis výsledku v původním jazyce

This paper presents two water-soluble fullerene nanomaterials (HexakisaminoC(60) and monoglucosamineC(60), which is called here JK39) that were developed and synthesized as non-viral siRNA transfection nanosystems. The developed two-step Bingel-Hirsch reaction enables the chemical modification of the fullerene scaffold with the desired bioactive fragments such as d-glucosamine while keeping the crucial positive charged ethylenediamine based malonate. The ESI-MS and C-13-NMR analyses of JK39 confirmed its high T-h symmetry, while X-ray photoelectron spectroscopy revealed the presence of nitrogen and oxygen-containing C-O or C-N bonds. The efficiency of both fullerenes as siRNA vehicles was tested in vitro using the prostate cancer cell line DU145 expressing the GFP protein. The HexakisaminoC(60) fullerene was an efficient siRNA transfection agent, and decreased the GFP fluorescence signal significantly in the DU145 cells. Surprisingly, the glycofullerene JK39 was inactive in the transfection experiments, probably due to its high zeta potential and the formation of an extremely stable complex with siRNA.

Název v anglickém jazyce

Towards water-soluble [60]fullerenes for the delivery of siRNA in a prostate cancer model

Popis výsledku anglicky

This paper presents two water-soluble fullerene nanomaterials (HexakisaminoC(60) and monoglucosamineC(60), which is called here JK39) that were developed and synthesized as non-viral siRNA transfection nanosystems. The developed two-step Bingel-Hirsch reaction enables the chemical modification of the fullerene scaffold with the desired bioactive fragments such as d-glucosamine while keeping the crucial positive charged ethylenediamine based malonate. The ESI-MS and C-13-NMR analyses of JK39 confirmed its high T-h symmetry, while X-ray photoelectron spectroscopy revealed the presence of nitrogen and oxygen-containing C-O or C-N bonds. The efficiency of both fullerenes as siRNA vehicles was tested in vitro using the prostate cancer cell line DU145 expressing the GFP protein. The HexakisaminoC(60) fullerene was an efficient siRNA transfection agent, and decreased the GFP fluorescence signal significantly in the DU145 cells. Surprisingly, the glycofullerene JK39 was inactive in the transfection experiments, probably due to its high zeta potential and the formation of an extremely stable complex with siRNA.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific reports

ISSN

2045-2322

e-ISSN

—

Svazek periodika

11

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

"Article Number:10565"

Kód UT WoS článku

000658822200002

EID výsledku v databázi Scopus

2-s2.0-85106296468