Highly repeatable and selective ultrahigh-performance supercritical fluid chromatography-Mass spectrometry interclass separation in lipidomic studies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F22%3A50019224" target="_blank" >RIV/62690094:18470/22:50019224 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0026265X22002041?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0026265X22002041?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.microc.2022.107376" target="_blank" >10.1016/j.microc.2022.107376</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Highly repeatable and selective ultrahigh-performance supercritical fluid chromatography-Mass spectrometry interclass separation in lipidomic studies

Popis výsledku v původním jazyce

Extensive research has recently established ultrahigh-performance supercritical fluid chromatography - mass spectrometry (UHPSFC/MS) coupling as an excellent tool for the fast and comprehensive characterization of lipids. However, the long-term instability of retention times using UHPSFC needs to be addressed, which is especially undesirable in lipidomic studies involving large clinical cohorts that are based on the comparison of lipid profiles between disease and control samples. In this work, we focused on conducting a detailed evaluation of the UHPSFC interclass separation of total lipid extracts for selective and repeatable lipidomic analysis. The selectivity of 9 chromatographic columns with different chemistry and the effect of particular chromatographic conditions on the lipid class resolution were carefully assessed. The most pronounced influence was found for the solvent type that was used as a modifier, which enabled the lipid class resolution to be finely adjusted. When propan-2-ol was added to methanol, a positive effect was observed for the resolution of mainly polar lipid classes and the intraclass resolution of lipid species was mildly suppressed; these were both favorable for the interclass separation used in this work. The optimized UHPSFC/MS method is based on a diol column and a gradient of methanol - propan-2-ol - water - ammonium formate modifier that provides the resolution of all lipid classes in brain samples within 7 min of analysis. This method has shown excellent retention times repeatability for the 555 injections in lipidomic study with the standard deviations below 0.012 min for all lipids. This demonstrates the ability of the optimized UHPSFC/MS method for highly selective and repeatable lipidomic analysis of biological samples.

Název v anglickém jazyce

Highly repeatable and selective ultrahigh-performance supercritical fluid chromatography-Mass spectrometry interclass separation in lipidomic studies

Popis výsledku anglicky

Extensive research has recently established ultrahigh-performance supercritical fluid chromatography - mass spectrometry (UHPSFC/MS) coupling as an excellent tool for the fast and comprehensive characterization of lipids. However, the long-term instability of retention times using UHPSFC needs to be addressed, which is especially undesirable in lipidomic studies involving large clinical cohorts that are based on the comparison of lipid profiles between disease and control samples. In this work, we focused on conducting a detailed evaluation of the UHPSFC interclass separation of total lipid extracts for selective and repeatable lipidomic analysis. The selectivity of 9 chromatographic columns with different chemistry and the effect of particular chromatographic conditions on the lipid class resolution were carefully assessed. The most pronounced influence was found for the solvent type that was used as a modifier, which enabled the lipid class resolution to be finely adjusted. When propan-2-ol was added to methanol, a positive effect was observed for the resolution of mainly polar lipid classes and the intraclass resolution of lipid species was mildly suppressed; these were both favorable for the interclass separation used in this work. The optimized UHPSFC/MS method is based on a diol column and a gradient of methanol - propan-2-ol - water - ammonium formate modifier that provides the resolution of all lipid classes in brain samples within 7 min of analysis. This method has shown excellent retention times repeatability for the 555 injections in lipidomic study with the standard deviations below 0.012 min for all lipids. This demonstrates the ability of the optimized UHPSFC/MS method for highly selective and repeatable lipidomic analysis of biological samples.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA20-12289S" target="_blank" >GA20-12289S: Spojení superkritické fluidní chromatografie a hmotnostní spektrometrie jako nový nástroj pro charakterizaci lipidů a polárních metabolitů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

MICROCHEMICAL JOURNAL

ISSN

0026-265X

e-ISSN

1095-9149

Svazek periodika

178

Číslo periodika v rámci svazku

July

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

"Article Number: 107376"

Kód UT WoS článku

000793743900003

EID výsledku v databázi Scopus

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