Ultrahigh-performance supercritical fluid chromatography for intraclass separation of lipids: Investigation of general principles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F22%3A50019225" target="_blank" >RIV/62690094:18470/22:50019225 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S002196732200173X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S002196732200173X?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2022.462975" target="_blank" >10.1016/j.chroma.2022.462975</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Ultrahigh-performance supercritical fluid chromatography for intraclass separation of lipids: Investigation of general principles

Popis výsledku v původním jazyce

Powerful chromatographic techniques are required for lipidomic analyses due to the extreme complex-ity of natural lipidomes. In the past few years, ultrahigh-performance supercritical fluid chromatography (UHPSFC) has proven to be a good alternative to conventional LC methods for comprehensive lipidomic analysis. The goal of this work was to study UHPSFC intraclass separation of lipids according to the fatty acyl composition. The effects of column chemistry, mobile phase composition and gradient, flow rate, back pressure, temperature, and column coupling on intraclass separation of lipids were widely inves-tigated and discussed. In general, UHPSFC exhibited interclass selectivity together with intraclass sepa-ration of lipids according to their total number of double bonds and acyl chain lengths. Moreover, sep-arations of diacylglycerol and lysophosphatidylcholines regioisomers were achieved in some cases. The nature of the stationary phase showed the most prominent effect on UHPSFC intraclass selectivity, while other chromatographic conditions were used for partial improvement in resolution of lipid species. An octadecyl-based stationary phase showed excellent separation of nonpolar lipid species, including tria-cylglycerol isobars; however, it provided poor peak shapes and limited retention time reproducibility for polar lipids. Diol-and 1-aminoanthracene-based columns provided the best inter-and intraclass resolu-tion of most lipids. The main benefit for UHPSFC separation of complex lipid samples is the combination of the acyl chain/double bond intraclass separation of lipids with excellent lipid class selectivity, which can facilitate mass spectrometry detection and quantitation of trace species without ion suppression ef-fects.(c) 2022 Elsevier B.V. All rights reserved.

Název v anglickém jazyce

Ultrahigh-performance supercritical fluid chromatography for intraclass separation of lipids: Investigation of general principles

Popis výsledku anglicky

Powerful chromatographic techniques are required for lipidomic analyses due to the extreme complex-ity of natural lipidomes. In the past few years, ultrahigh-performance supercritical fluid chromatography (UHPSFC) has proven to be a good alternative to conventional LC methods for comprehensive lipidomic analysis. The goal of this work was to study UHPSFC intraclass separation of lipids according to the fatty acyl composition. The effects of column chemistry, mobile phase composition and gradient, flow rate, back pressure, temperature, and column coupling on intraclass separation of lipids were widely inves-tigated and discussed. In general, UHPSFC exhibited interclass selectivity together with intraclass sepa-ration of lipids according to their total number of double bonds and acyl chain lengths. Moreover, sep-arations of diacylglycerol and lysophosphatidylcholines regioisomers were achieved in some cases. The nature of the stationary phase showed the most prominent effect on UHPSFC intraclass selectivity, while other chromatographic conditions were used for partial improvement in resolution of lipid species. An octadecyl-based stationary phase showed excellent separation of nonpolar lipid species, including tria-cylglycerol isobars; however, it provided poor peak shapes and limited retention time reproducibility for polar lipids. Diol-and 1-aminoanthracene-based columns provided the best inter-and intraclass resolu-tion of most lipids. The main benefit for UHPSFC separation of complex lipid samples is the combination of the acyl chain/double bond intraclass separation of lipids with excellent lipid class selectivity, which can facilitate mass spectrometry detection and quantitation of trace species without ion suppression ef-fects.(c) 2022 Elsevier B.V. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

<a href="/cs/project/GA20-12289S" target="_blank" >GA20-12289S: Spojení superkritické fluidní chromatografie a hmotnostní spektrometrie jako nový nástroj pro charakterizaci lipidů a polárních metabolitů</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

1873-3778

Svazek periodika

1670

Číslo periodika v rámci svazku

May

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

"Article Number: 462975"

Kód UT WoS článku

000789608700014

EID výsledku v databázi Scopus

2-s2.0-85126523436