Column selection for improvement of the UHPLC lipidomic analysis
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F23%3A00573205" target="_blank" >RIV/61388963:_____/23:00573205 - isvavai.cz</a>
Výsledek na webu
<a href="https://hdl.handle.net/11104/0343658" target="_blank" >https://hdl.handle.net/11104/0343658</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Column selection for improvement of the UHPLC lipidomic analysis
Popis výsledku v původním jazyce
Reversed-phase UHPLC/MS is a widely used lipidomic strategy that allows the separation of the lipid species based on their equivalent carbon number (ECN - the number of carbons minus two times the number of double bonds of all fatty acids). Lipid classes as well as fatty acid compositions are determined using fragments in MSn spectra. Retention time has to correspond with identifying lipid species, so the knowledge of retention dependences significantly enhances the certainty of identification. Increasing chromatographic peak resolution allows identifying more lipid species, and short run times are essential for analyzing large sets of samples in lipidomics. Reducing the slope of the gradient is not a suitable solution for increasing resolution, so selecting a suitable stationary phase is critical. In this study, four C18 columns with sub-2-micrometer particles were compared using a mixture of 71 lipid standards from 16 lipid classes. n
Název v anglickém jazyce
Column selection for improvement of the UHPLC lipidomic analysis
Popis výsledku anglicky
Reversed-phase UHPLC/MS is a widely used lipidomic strategy that allows the separation of the lipid species based on their equivalent carbon number (ECN - the number of carbons minus two times the number of double bonds of all fatty acids). Lipid classes as well as fatty acid compositions are determined using fragments in MSn spectra. Retention time has to correspond with identifying lipid species, so the knowledge of retention dependences significantly enhances the certainty of identification. Increasing chromatographic peak resolution allows identifying more lipid species, and short run times are essential for analyzing large sets of samples in lipidomics. Reducing the slope of the gradient is not a suitable solution for increasing resolution, so selecting a suitable stationary phase is critical. In this study, four C18 columns with sub-2-micrometer particles were compared using a mixture of 71 lipid standards from 16 lipid classes. n
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
<a href="/cs/project/LX22NPO5104" target="_blank" >LX22NPO5104: Národní institut pro výzkum metabolických a kardiovaskulárních onemocnění</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů