Alpha-class glutathione S-transferases involved in the detoxification of aflatoxin B1 in ducklings

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F23%3A50020271" target="_blank" >RIV/62690094:18470/23:50020271 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0278691523000844?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0278691523000844?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.fct.2023.113682" target="_blank" >10.1016/j.fct.2023.113682</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Alpha-class glutathione S-transferases involved in the detoxification of aflatoxin B1 in ducklings

Popis výsledku v původním jazyce

The objective of this study was to identify the key glutathione S-transferase (GST) isozymes involved in the detoxification of Aflatoxin B1 (AFB1) in ducks&apos; primary hepatocytes. The full-length cDNA encoding the 10 GST isozymes (GST, GST3, GSTM3, MGST1, MGST2, MGST3, GSTK1, GSTT1, GSTO1 and GSTZ1) were isolated/synthesized from ducks&apos; liver and cloned into the pcDNA3.1(+) vector. The results showed that pcDNA3.1(+)-GSTs plasmids were successfully transferred into the ducks&apos; primary hepatocytes and the mRNA of the 10 GST isozymes were overexpressed by 1.9–3274.7 times. Compared to the control, 75 μg/L (IC30) or 150 μg/L (IC50) AFB1 treatment reduced the cell viability by 30.0–50.0% and increased the LDH activity by 19.8–58.2% in the ducks&apos; primary hepatocytes. Notably, the AFB1–induced changes in cell viability and LDH activity were mitigated by overexpression of GST and GST3. Compared to the cells treated with AFB1, exo-AFB1-8,9-epoxide (AFBO)–GSH, as the major detoxified product of AFB1, was increased in the cells overexpression of GST and GST3. Moreover, the sequences, phylogenetic and domain analysis revealed that the GST and GST3 were orthologous to Meleagris gallopavo GSTA3 and GSTA4. In conclusion, this study found that the ducks&apos; GST and GST3 were orthologous to Meleagris gallopavo GSTA3 and GSTA4, which were involved in the detoxification of AFB1 in ducks’ primary hepatocytes. © 2023 Elsevier Ltd

Název v anglickém jazyce

Alpha-class glutathione S-transferases involved in the detoxification of aflatoxin B1 in ducklings

Popis výsledku anglicky

The objective of this study was to identify the key glutathione S-transferase (GST) isozymes involved in the detoxification of Aflatoxin B1 (AFB1) in ducks&apos; primary hepatocytes. The full-length cDNA encoding the 10 GST isozymes (GST, GST3, GSTM3, MGST1, MGST2, MGST3, GSTK1, GSTT1, GSTO1 and GSTZ1) were isolated/synthesized from ducks&apos; liver and cloned into the pcDNA3.1(+) vector. The results showed that pcDNA3.1(+)-GSTs plasmids were successfully transferred into the ducks&apos; primary hepatocytes and the mRNA of the 10 GST isozymes were overexpressed by 1.9–3274.7 times. Compared to the control, 75 μg/L (IC30) or 150 μg/L (IC50) AFB1 treatment reduced the cell viability by 30.0–50.0% and increased the LDH activity by 19.8–58.2% in the ducks&apos; primary hepatocytes. Notably, the AFB1–induced changes in cell viability and LDH activity were mitigated by overexpression of GST and GST3. Compared to the cells treated with AFB1, exo-AFB1-8,9-epoxide (AFBO)–GSH, as the major detoxified product of AFB1, was increased in the cells overexpression of GST and GST3. Moreover, the sequences, phylogenetic and domain analysis revealed that the GST and GST3 were orthologous to Meleagris gallopavo GSTA3 and GSTA4. In conclusion, this study found that the ducks&apos; GST and GST3 were orthologous to Meleagris gallopavo GSTA3 and GSTA4, which were involved in the detoxification of AFB1 in ducks’ primary hepatocytes. © 2023 Elsevier Ltd

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30108 - Toxicology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Food and chemical toxicology

ISSN

0278-6915

e-ISSN

1873-6351

Svazek periodika

174

Číslo periodika v rámci svazku

April

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

"Article number: 113682"

Kód UT WoS článku

000949780300001

EID výsledku v databázi Scopus

2-s2.0-85150058386