Cloning of CAT genes in Satsuma mandarin and their expression characteristics in response to environmental stress and arbuscular mycorrhizal fungi

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F24%3A50021445" target="_blank" >RIV/62690094:18470/24:50021445 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s00299-024-03218-7" target="_blank" >https://link.springer.com/article/10.1007/s00299-024-03218-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00299-024-03218-7" target="_blank" >10.1007/s00299-024-03218-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cloning of CAT genes in Satsuma mandarin and their expression characteristics in response to environmental stress and arbuscular mycorrhizal fungi

Popis výsledku v původním jazyce

Catalase (CAT), a tetrameric heme-containing enzyme, removes hydrogen peroxide (H2O2) to maintain low oxidativedamage in plants exposed to environmental stress. This study aimed to clone CAT genes from Citrus sinensis cv. “Oita4” and analyze their expression patterns in response to environmental stress, exogenous abscisic acid (ABA), and arbuscularmycorrhizal fungal inoculation. Two CAT genes, CitCAT1 (NCBI accession: PP067858) and CitCAT2 (NCBI accession:PP061394) were cloned, and the open reading frames of their proteins were 1479 bp and 1539 bp, respectively, each encoding492 and 512 amino acids predicted to be localized in the peroxisome, with CitCAT1 being a stable hydrophilic protein andCitCAT2 being an unstable hydrophilic protein. The similarity of their amino acid sequences reached 83.24%, and the twogenes were distantly related. Both genes were expressed in stems, leaves, flowers, and fruits, accompanied by the highestexpression in mature leaves. In addition, CitCAT1 expression was mainly up-regulated by high temperatures (37 °C), exogenousABA, and PEG stress within a short period of time, whereas CitCAT2 expression was up-regulated by exogenous ABAand low-temperature (4 °C) stress. Low temperatures (0 °C) for 12 h just up-regulated CitCAT2 expression in Diversisporaversiformis-inoculated plants, and D. versiformis inoculation up-regulated CitCAT2 expression, along with lower hydrogenperoxide and malondialdehyde levels in mycorrhizal plants at low temperatures. It is concluded that CitCAT2 has an importantrole in resistance to low temperatures as well as mycorrhizal enhancement of host resistance to low temperatures.

Název v anglickém jazyce

Cloning of CAT genes in Satsuma mandarin and their expression characteristics in response to environmental stress and arbuscular mycorrhizal fungi

Popis výsledku anglicky

Catalase (CAT), a tetrameric heme-containing enzyme, removes hydrogen peroxide (H2O2) to maintain low oxidativedamage in plants exposed to environmental stress. This study aimed to clone CAT genes from Citrus sinensis cv. “Oita4” and analyze their expression patterns in response to environmental stress, exogenous abscisic acid (ABA), and arbuscularmycorrhizal fungal inoculation. Two CAT genes, CitCAT1 (NCBI accession: PP067858) and CitCAT2 (NCBI accession:PP061394) were cloned, and the open reading frames of their proteins were 1479 bp and 1539 bp, respectively, each encoding492 and 512 amino acids predicted to be localized in the peroxisome, with CitCAT1 being a stable hydrophilic protein andCitCAT2 being an unstable hydrophilic protein. The similarity of their amino acid sequences reached 83.24%, and the twogenes were distantly related. Both genes were expressed in stems, leaves, flowers, and fruits, accompanied by the highestexpression in mature leaves. In addition, CitCAT1 expression was mainly up-regulated by high temperatures (37 °C), exogenousABA, and PEG stress within a short period of time, whereas CitCAT2 expression was up-regulated by exogenous ABAand low-temperature (4 °C) stress. Low temperatures (0 °C) for 12 h just up-regulated CitCAT2 expression in Diversisporaversiformis-inoculated plants, and D. versiformis inoculation up-regulated CitCAT2 expression, along with lower hydrogenperoxide and malondialdehyde levels in mycorrhizal plants at low temperatures. It is concluded that CitCAT2 has an importantrole in resistance to low temperatures as well as mycorrhizal enhancement of host resistance to low temperatures.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10611 - Plant sciences, botany

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Plant Cell Reports

ISSN

0721-7714

e-ISSN

1432-203X

Svazek periodika

43

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

"Article number: 123"

Kód UT WoS článku

001205943600002

EID výsledku v databázi Scopus

2-s2.0-85190765118