?Mycobacterium avium subsp. Hominissuis? in Neck Lymph Nodes of Children and their Enviroment Examined by Culture and Triplex Quantitative Real-Time PCR

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F11%3A%230001248" target="_blank" >RIV/65269705:_____/11:#0001248 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1128/JCM.00802-10" target="_blank" >http://dx.doi.org/10.1128/JCM.00802-10</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/JCM.00802-10" target="_blank" >10.1128/JCM.00802-10</a>

Jazyk výsledku

angličtina

Název v původním jazyce

?Mycobacterium avium subsp. Hominissuis? in Neck Lymph Nodes of Children and their Enviroment Examined by Culture and Triplex Quantitative Real-Time PCR

Popis výsledku v původním jazyce

Mycobacterium avium subsp. hominissuis? often causes cervical lymphadenitis in children; its prompt and accurate identification enables adequate therapy, tracing, and prevention. The aims of this study were to determine the causative agent of lymphadenitis using culture, PCR, and triplex quantitative real-time PCR (qPCR) methods with DNA directly isolated from tissue, as well as to identify possible sources of infection from the environment. We confirmed the diagnoses by detecting M. avium subsp. hominissuis using qPCR with DNA directly isolated from lymph node biopsy specimens of two patients. In order to trace the source of infection from the environment, a method of DNA isolation from soil and other environmental samples, such as dust, cobwebs, andcompost, was developed. The triplex qPCR examination revealed the presence of M. avium subsp. hominissuis in a high proportion of the environmental samples (42.8% in the first patient?s house and 47.6% in the second patient?s house). Both

Název v anglickém jazyce

?Mycobacterium avium subsp. Hominissuis? in Neck Lymph Nodes of Children and their Enviroment Examined by Culture and Triplex Quantitative Real-Time PCR

Popis výsledku anglicky

Mycobacterium avium subsp. hominissuis? often causes cervical lymphadenitis in children; its prompt and accurate identification enables adequate therapy, tracing, and prevention. The aims of this study were to determine the causative agent of lymphadenitis using culture, PCR, and triplex quantitative real-time PCR (qPCR) methods with DNA directly isolated from tissue, as well as to identify possible sources of infection from the environment. We confirmed the diagnoses by detecting M. avium subsp. hominissuis using qPCR with DNA directly isolated from lymph node biopsy specimens of two patients. In order to trace the source of infection from the environment, a method of DNA isolation from soil and other environmental samples, such as dust, cobwebs, andcompost, was developed. The triplex qPCR examination revealed the presence of M. avium subsp. hominissuis in a high proportion of the environmental samples (42.8% in the first patient?s house and 47.6% in the second patient?s house). Both

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FN - Epidemiologie, infekční nemoci a klinická imunologie

OECD FORD obor

—

Projekt

—

Návaznosti

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Clinical Microbiology

ISSN

0095-1137

e-ISSN

—

Svazek periodika

49

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

167-172

Kód UT WoS článku

000285787100022

EID výsledku v databázi Scopus

—