Assessment of antibodies against surface and outer membrane proteins of Anaplasma phagocytophilum in Lyme borreliosis and tick-borne encephalitis paediatric patients

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F16%3A00065595" target="_blank" >RIV/65269705:_____/16:00065595 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/16:00091214

Výsledek na webu

<a href="http://dx.doi.org/10.1017/S0950268816000972" target="_blank" >http://dx.doi.org/10.1017/S0950268816000972</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S0950268816000972" target="_blank" >10.1017/S0950268816000972</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Assessment of antibodies against surface and outer membrane proteins of Anaplasma phagocytophilum in Lyme borreliosis and tick-borne encephalitis paediatric patients

Popis výsledku v původním jazyce

To examine evidence of positive antibodies against immunogenic proteins of Anaplasma phagocytophilum in patients with other tick-borne infections and to diagnose possible co-infections, 412 serum specimens were tested by immunoblotting using three specific Anaplasma antigens: surface proteins p44 and Asp62 and outer membrane protein A (OmpA). In total, 284 serum samples from children with Lyme borreliosis and 12 serum samples from children with tick-borne encephalitis were tested. Sera from patients with viral aseptic meningitis (n = 47) and from blood donors (n = 69) were used as controls. Among all serum specimens from patients with tick-borne infections submitted for this study, six samples (20%) showed positive IgM reactions and seven samples (24%) were IgG positive for A. phagocytophilum by immunoblot. Borderline reactivity was found in 30 samples (1014%) for IgM and 36 samples (122%) for IgG. The difference between patients and blood donors was statistically significant for IgM (P = 0006) and for IgG (P = 00007) antibodies. A statistically significant result was obtained for IgG (P = 002) but not for IgM between patients and children with aseptic meningitis. Immunoblot using three specific antigens provides novel information about the positivity of antibodies to A. phagocytophilum in children with other tick-borne infections. Taking into account clinical and laboratory findings of children despite antibody positivity, no case of human granulocytic anaplasmosis was demonstrated.

Název v anglickém jazyce

Assessment of antibodies against surface and outer membrane proteins of Anaplasma phagocytophilum in Lyme borreliosis and tick-borne encephalitis paediatric patients

Popis výsledku anglicky

To examine evidence of positive antibodies against immunogenic proteins of Anaplasma phagocytophilum in patients with other tick-borne infections and to diagnose possible co-infections, 412 serum specimens were tested by immunoblotting using three specific Anaplasma antigens: surface proteins p44 and Asp62 and outer membrane protein A (OmpA). In total, 284 serum samples from children with Lyme borreliosis and 12 serum samples from children with tick-borne encephalitis were tested. Sera from patients with viral aseptic meningitis (n = 47) and from blood donors (n = 69) were used as controls. Among all serum specimens from patients with tick-borne infections submitted for this study, six samples (20%) showed positive IgM reactions and seven samples (24%) were IgG positive for A. phagocytophilum by immunoblot. Borderline reactivity was found in 30 samples (1014%) for IgM and 36 samples (122%) for IgG. The difference between patients and blood donors was statistically significant for IgM (P = 0006) and for IgG (P = 00007) antibodies. A statistically significant result was obtained for IgG (P = 002) but not for IgM between patients and children with aseptic meningitis. Immunoblot using three specific antigens provides novel information about the positivity of antibodies to A. phagocytophilum in children with other tick-borne infections. Taking into account clinical and laboratory findings of children despite antibody positivity, no case of human granulocytic anaplasmosis was demonstrated.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FN - Epidemiologie, infekční nemoci a klinická imunologie

OECD FORD obor

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Projekt

—

Návaznosti

N - Vyzkumna aktivita podporovana z neverejnych zdroju

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Epidemiology and Infection

ISSN

0950-2688

e-ISSN

—

Svazek periodika

144

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

2597-2604

Kód UT WoS článku

000382174600015

EID výsledku v databázi Scopus

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