Transcriptomic profile of cell cycle progression genes in human ovarian granulosa cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F19%3A00070866" target="_blank" >RIV/65269705:_____/19:00070866 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/19:00113002

Výsledek na webu

<a href="https://www.biolifesas.org/biolife/2019/02/19/insight-into-nuclear-cytoplasmic-shuttling-as-a-developmental-and-differentiational-capability-of-cells-in-primary-culture-models/" target="_blank" >https://www.biolifesas.org/biolife/2019/02/19/insight-into-nuclear-cytoplasmic-shuttling-as-a-developmental-and-differentiational-capability-of-cells-in-primary-culture-models/</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Transcriptomic profile of cell cycle progression genes in human ovarian granulosa cells

Popis výsledku v původním jazyce

The ovarian granulosa cells (GCs) that form the structure of follicle undergo substantial modification during the various stages of human folliculogenesis. These modifications include morphological changes, accompanied by differential expression of genes, encoding proteins which are mainly involved in cell growth, proliferation and differentiation. Recent data bring a new insight into the aspects of GCs&apos; stem-like specificity and plasticity, enabling their prolonged proliferation and differentiation into other cell types. This manuscript focuses attention on emerging alterations during GC cell cycle - a series of biochemical and biophysical changes within the cell. Human GCs were collected from follicles of women set to undergo intracytoplasmic sperm injection procedure, as a part of remnant follicular fluid. The cells were primarily cultured for 30 days. Throughout this time, we observed the prominent change in cell morphology from epithelial-like to fibroblast-like, suggesting differentiation to other cell types. Additionally, at days 1, 7, 15 and 30, the RNA was isolated for molecular assays. Using Affymetrie (R) Human Genome U219 Array, we found 2579 human transcripts that were differentially expressed in GCs. From these genes, we extracted 582 Gene Ontology Biological Process (GO BP) Terms and 45 KEGG pathways, among which we investigated transcripts belonging to four GO BPs associated ith cell proliferation: &quot;cell cycle phase transition&quot;, &quot;Gl/S phase transition&quot;, G2/M phase transition&quot; and &quot;cell cycle checkpoint&quot;. Microarray results were validated by RT-qPCR. Increased expression of all the genes studied indicated that increase in GC proliferation during long-term in vitro culture is orchestrated by the up-regulation of genes related to cell cycle control. Furthermore, observed changes in cell morphology may be regulated by a presented set of genes, leading to the induction of pathways specific for sternness plasticity and transdifferentiation in vitro.

Název v anglickém jazyce

Transcriptomic profile of cell cycle progression genes in human ovarian granulosa cells

Popis výsledku anglicky

The ovarian granulosa cells (GCs) that form the structure of follicle undergo substantial modification during the various stages of human folliculogenesis. These modifications include morphological changes, accompanied by differential expression of genes, encoding proteins which are mainly involved in cell growth, proliferation and differentiation. Recent data bring a new insight into the aspects of GCs&apos; stem-like specificity and plasticity, enabling their prolonged proliferation and differentiation into other cell types. This manuscript focuses attention on emerging alterations during GC cell cycle - a series of biochemical and biophysical changes within the cell. Human GCs were collected from follicles of women set to undergo intracytoplasmic sperm injection procedure, as a part of remnant follicular fluid. The cells were primarily cultured for 30 days. Throughout this time, we observed the prominent change in cell morphology from epithelial-like to fibroblast-like, suggesting differentiation to other cell types. Additionally, at days 1, 7, 15 and 30, the RNA was isolated for molecular assays. Using Affymetrie (R) Human Genome U219 Array, we found 2579 human transcripts that were differentially expressed in GCs. From these genes, we extracted 582 Gene Ontology Biological Process (GO BP) Terms and 45 KEGG pathways, among which we investigated transcripts belonging to four GO BPs associated ith cell proliferation: &quot;cell cycle phase transition&quot;, &quot;Gl/S phase transition&quot;, G2/M phase transition&quot; and &quot;cell cycle checkpoint&quot;. Microarray results were validated by RT-qPCR. Increased expression of all the genes studied indicated that increase in GC proliferation during long-term in vitro culture is orchestrated by the up-regulation of genes related to cell cycle control. Furthermore, observed changes in cell morphology may be regulated by a presented set of genes, leading to the induction of pathways specific for sternness plasticity and transdifferentiation in vitro.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

30105 - Physiology (including cytology)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biological Regulators and Homeostatic Agents

ISSN

0393-974X

e-ISSN

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Svazek periodika

33

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

IT - Italská republika

Počet stran výsledku

13

Strana od-do

39-51

Kód UT WoS článku

000464697500005

EID výsledku v databázi Scopus

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