“Cell cycle process”, “cell division” and “cell proliferation” belong to ontology groups highly regulated during long–term culture of porcine oviductal epithelial cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F19%3A00071125" target="_blank" >RIV/65269705:_____/19:00071125 - isvavai.cz</a>

Výsledek na webu

<a href="https://content.sciendo.com/view/journals/acb/7/1/article-p15.xml" target="_blank" >https://content.sciendo.com/view/journals/acb/7/1/article-p15.xml</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/acb-2019-0003" target="_blank" >10.2478/acb-2019-0003</a>

Jazyk výsledku

angličtina

Název v původním jazyce

“Cell cycle process”, “cell division” and “cell proliferation” belong to ontology groups highly regulated during long–term culture of porcine oviductal epithelial cells

Popis výsledku v původním jazyce

Morphological and biochemical changes in the cells surrounding the oocyte seem to be extremely important in an effective fertilization process. Thanks to advanced cell culture techniques, as well as biochemical and bioinformatics analyses, we can partly imitate the phenomena occurring in the living organism. Previous studies showed a possibility of short - and long - term OEC in vitro cultivation, during which these cells have shown to have significant proliferation and expression of genes responsible for differentiation. Our research was aimed at maintaining a culture of porcine oviduct epithelial cells and analyzing their gene expression profile. The study employed cross-bred gilts at the age of about 9 months, obtained from commercial herds. With the use of Affymetrix(R) Porcine Gene 1.1 ST Array Strip, we have examined the expression of 12257 transcripts. Genes with fold change higher than abs (2) and with corrected p-value lower than 0.05 were considered as differentially expressed. We chose 20 genes with the most marked expression (10 up - regulated, 10 down - regulated) for further investigation in the context of literature sources. These genes belonged to three ontological groups: &quot;cell cycle process&quot;, &quot;cell division&quot; and &quot;cell proliferation&quot;. The results obtained from these studies may be the basis for further molecular analyses.

Název v anglickém jazyce

“Cell cycle process”, “cell division” and “cell proliferation” belong to ontology groups highly regulated during long–term culture of porcine oviductal epithelial cells

Popis výsledku anglicky

Morphological and biochemical changes in the cells surrounding the oocyte seem to be extremely important in an effective fertilization process. Thanks to advanced cell culture techniques, as well as biochemical and bioinformatics analyses, we can partly imitate the phenomena occurring in the living organism. Previous studies showed a possibility of short - and long - term OEC in vitro cultivation, during which these cells have shown to have significant proliferation and expression of genes responsible for differentiation. Our research was aimed at maintaining a culture of porcine oviduct epithelial cells and analyzing their gene expression profile. The study employed cross-bred gilts at the age of about 9 months, obtained from commercial herds. With the use of Affymetrix(R) Porcine Gene 1.1 ST Array Strip, we have examined the expression of 12257 transcripts. Genes with fold change higher than abs (2) and with corrected p-value lower than 0.05 were considered as differentially expressed. We chose 20 genes with the most marked expression (10 up - regulated, 10 down - regulated) for further investigation in the context of literature sources. These genes belonged to three ontological groups: &quot;cell cycle process&quot;, &quot;cell division&quot; and &quot;cell proliferation&quot;. The results obtained from these studies may be the basis for further molecular analyses.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

10601 - Cell biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Medical Journal of Cell Biology

ISSN

2544-3577

e-ISSN

—

Svazek periodika

7

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

10

Strana od-do

15-24

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85069968325