Study of PIWI-interacting RNAs in the pathology of glioblastoma: a new level of glioblastoma stem cell regulation?

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F21%3A00074518" target="_blank" >RIV/65269705:_____/21:00074518 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/21:00120190

Výsledek na webu

<a href="http://rimononline.in.ua/neurosurgery2021/eng#rec326396620" target="_blank" >http://rimononline.in.ua/neurosurgery2021/eng#rec326396620</a>

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Study of PIWI-interacting RNAs in the pathology of glioblastoma: a new level of glioblastoma stem cell regulation?

Popis výsledku v původním jazyce

Background: Glioblastoma (GBM) is the most common malignant brain tumor of astrocytic origin. Despite the radical therapy, relapse is a common and relatively early event, with the major mechanism possibly being the presence of glioblastoma stem cells (GSC), able to withstand the therapy and to establish new GBM deposits. PIWI-interacting RNAs (piRNA) may have a key role in GSC biology, which are physiologically responsible for the genome stability maintenance in germ and stem cells. Nevertheless, their dysregulation was observed in cancers, including GBM. Identification of GSC-specific piRNA molecules could lead to distinguishing GSC from their differentiated counterparts (non-GSC), enabling their therapeutic targeting. Material and methodology: Fresh dissociated GBM tissue divided into two aliquots was used for the derivation of GSC and non-GSC primary cultures and cultivation in serum-free or serum-containing medium, respectively. Paired cultures were selected for sequencing (NGS) based on their neural stem marker (CD133 and Sox2) expression, the ability to propagate tumors in immunodeficient mice and the ability to differentiate. Library preparation was performed using NEXTFLEX Small RNA-Seq Kit v3, with prolonged 3&apos; adaptor ligation and the treatment of samples with blocking oligonucleotides for YRNA and tRNA fragments for better piRNA capture. NGS was performed in NextSeq 500 (Illumina). Results: Twelve paired cultures were selected according to CD133 expression (average expression in GSC = 67.5%, GSC ranged 22.5-99.2% of CD133+ cells, non-GSC ranged 0.14-1.17% of CD133+ cells), Sox2 expression (average expression in GSC = 51.2%, GSC ranged 1.34-97.2% of Sox2+ cells, non-GSC ranged 0.23-2.96% of Sox2+ cells), and the ability of GSC to form tumors in immunodeficient mice and to differentiate. NGS results are currently being statistically evaluated. Summary: Dysregulated GSC-specific piRNAs can present a promising tool for the identification and therapeutic targeting of GSC which could significantly alter the prognosis of GBM patients.

Název v anglickém jazyce

Study of PIWI-interacting RNAs in the pathology of glioblastoma: a new level of glioblastoma stem cell regulation?

Popis výsledku anglicky

Background: Glioblastoma (GBM) is the most common malignant brain tumor of astrocytic origin. Despite the radical therapy, relapse is a common and relatively early event, with the major mechanism possibly being the presence of glioblastoma stem cells (GSC), able to withstand the therapy and to establish new GBM deposits. PIWI-interacting RNAs (piRNA) may have a key role in GSC biology, which are physiologically responsible for the genome stability maintenance in germ and stem cells. Nevertheless, their dysregulation was observed in cancers, including GBM. Identification of GSC-specific piRNA molecules could lead to distinguishing GSC from their differentiated counterparts (non-GSC), enabling their therapeutic targeting. Material and methodology: Fresh dissociated GBM tissue divided into two aliquots was used for the derivation of GSC and non-GSC primary cultures and cultivation in serum-free or serum-containing medium, respectively. Paired cultures were selected for sequencing (NGS) based on their neural stem marker (CD133 and Sox2) expression, the ability to propagate tumors in immunodeficient mice and the ability to differentiate. Library preparation was performed using NEXTFLEX Small RNA-Seq Kit v3, with prolonged 3&apos; adaptor ligation and the treatment of samples with blocking oligonucleotides for YRNA and tRNA fragments for better piRNA capture. NGS was performed in NextSeq 500 (Illumina). Results: Twelve paired cultures were selected according to CD133 expression (average expression in GSC = 67.5%, GSC ranged 22.5-99.2% of CD133+ cells, non-GSC ranged 0.14-1.17% of CD133+ cells), Sox2 expression (average expression in GSC = 51.2%, GSC ranged 1.34-97.2% of Sox2+ cells, non-GSC ranged 0.23-2.96% of Sox2+ cells), and the ability of GSC to form tumors in immunodeficient mice and to differentiate. NGS results are currently being statistically evaluated. Summary: Dysregulated GSC-specific piRNAs can present a promising tool for the identification and therapeutic targeting of GSC which could significantly alter the prognosis of GBM patients.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

30212 - Surgery

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů