Three-dimensional Biomaterials as a Tool for In Vitro Culture of Leukemic B Cells
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F21%3A00075130" target="_blank" >RIV/65269705:_____/21:00075130 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.lfhk.cuni.cz/getattachment/Veda,-zahranici/Vedecka-setkani/Mezinarodni-konference-studentu-DSP/Archiv-mezinarodnich-konferenci-studentu-DSP/FINAL-SBORNIK-2021_2.pdf.aspx/?lang=en-GB" target="_blank" >https://www.lfhk.cuni.cz/getattachment/Veda,-zahranici/Vedecka-setkani/Mezinarodni-konference-studentu-DSP/Archiv-mezinarodnich-konferenci-studentu-DSP/FINAL-SBORNIK-2021_2.pdf.aspx/?lang=en-GB</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Three-dimensional Biomaterials as a Tool for In Vitro Culture of Leukemic B Cells
Popis výsledku v původním jazyce
Chronic lymphocytic leukemia (CLL) is the most common adult leukemia in Caucasians. However, mechanisms underlying the disease development still have not been fully elucidated. To unravel the machinery of neoplastic processes in CLL cells, in vitro culture is inevitable. Construction of a suitable microenvironmentlike in vitro platform therefore represents an important goal in CLL research. An authentic model of CLL can be, for example, achieved by utilization of three-dimensional biomaterials. In our study, we focused on use of porous scaffolds and gelling substances for the growth of cells which are naturally present in blood marrow of CLL patients, i.e., B cells (CLL cells) and bone marrow stromal cells. We observed that P(HEMA-co-AEMA)-RGDS hydrogels, collagen scaffolds, and CMC-PEG gels are biocompatible materials, which enable long-term 3D in vitro culture of HS5, M2-10B4, MEC-1, and HG-3 cell lines and short-term culture of primary CLL cells. While further optimizations of the culture systems are necessary, our methodology and results already represent an important step toward developing a 3D model of CLL.
Název v anglickém jazyce
Three-dimensional Biomaterials as a Tool for In Vitro Culture of Leukemic B Cells
Popis výsledku anglicky
Chronic lymphocytic leukemia (CLL) is the most common adult leukemia in Caucasians. However, mechanisms underlying the disease development still have not been fully elucidated. To unravel the machinery of neoplastic processes in CLL cells, in vitro culture is inevitable. Construction of a suitable microenvironmentlike in vitro platform therefore represents an important goal in CLL research. An authentic model of CLL can be, for example, achieved by utilization of three-dimensional biomaterials. In our study, we focused on use of porous scaffolds and gelling substances for the growth of cells which are naturally present in blood marrow of CLL patients, i.e., B cells (CLL cells) and bone marrow stromal cells. We observed that P(HEMA-co-AEMA)-RGDS hydrogels, collagen scaffolds, and CMC-PEG gels are biocompatible materials, which enable long-term 3D in vitro culture of HS5, M2-10B4, MEC-1, and HG-3 cell lines and short-term culture of primary CLL cells. While further optimizations of the culture systems are necessary, our methodology and results already represent an important step toward developing a 3D model of CLL.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
30204 - Oncology
Návaznosti výsledku
Projekt
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Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů