Effective NPM1 plasmid standards selection for minimal/measurable residual disease monitoring in acute myeloid leukemia

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F22%3A00076175" target="_blank" >RIV/65269705:_____/22:00076175 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/22:00126108

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s11033-022-07363-8" target="_blank" >https://link.springer.com/article/10.1007/s11033-022-07363-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11033-022-07363-8" target="_blank" >10.1007/s11033-022-07363-8</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effective NPM1 plasmid standards selection for minimal/measurable residual disease monitoring in acute myeloid leukemia

Popis výsledku v původním jazyce

Background NPM1 plasmid standards are required for absolute quantification of minimal residual disease in acute myeloid leukemia patients. The standards are usually obtained, next to commercially constructed gene fragments, from transgenic bacteria colonies. However, this procedure is laborious and very time consuming. Methods and results We have developed a PCR method that speeds up, simplifies, and streamlines the process of preparing NPM1 plasmid standards. The method is based on a combination of three primers, two surrounding the usual NPM1 mutation position and one over the mutation site. With this method, we were able to clearly distinguish plasmids with at least 15 different NPM1 mutations from the wild-type NPM1 plasmid. Conclusions With the new approach, preparing NPM1 plasmid standards is easier, identifying NPM1-positive colonies is possible in less than a day and moreover, for a lower price than commercially constructed gene fragments.

Název v anglickém jazyce

Effective NPM1 plasmid standards selection for minimal/measurable residual disease monitoring in acute myeloid leukemia

Popis výsledku anglicky

Background NPM1 plasmid standards are required for absolute quantification of minimal residual disease in acute myeloid leukemia patients. The standards are usually obtained, next to commercially constructed gene fragments, from transgenic bacteria colonies. However, this procedure is laborious and very time consuming. Methods and results We have developed a PCR method that speeds up, simplifies, and streamlines the process of preparing NPM1 plasmid standards. The method is based on a combination of three primers, two surrounding the usual NPM1 mutation position and one over the mutation site. With this method, we were able to clearly distinguish plasmids with at least 15 different NPM1 mutations from the wild-type NPM1 plasmid. Conclusions With the new approach, preparing NPM1 plasmid standards is easier, identifying NPM1-positive colonies is possible in less than a day and moreover, for a lower price than commercially constructed gene fragments.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Biology Reports

ISSN

0301-4851

e-ISSN

1573-4978

Svazek periodika

49

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

8169-8172

Kód UT WoS článku

000812606600010

EID výsledku v databázi Scopus

2-s2.0-85132138417