Exploring DNA Methylation Patterns in the Core Genome of Klebsiella pneumoniae

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F24%3A00080450" target="_blank" >RIV/65269705:_____/24:00080450 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26220/24:PU151993

Výsledek na webu

<a href="https://link.springer.com/chapter/10.1007/978-3-031-64636-2_11" target="_blank" >https://link.springer.com/chapter/10.1007/978-3-031-64636-2_11</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-3-031-64636-2_11" target="_blank" >10.1007/978-3-031-64636-2_11</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Exploring DNA Methylation Patterns in the Core Genome of Klebsiella pneumoniae

Popis výsledku v původním jazyce

In recent years, sequencing has become easily accessible and widespread, revolutionizing our ability to study complex genetic information. Thus, it is possible to analyze complex bacterial populations and investigate relationships between individual strains. Methods such as core genome multilocus sequence typing (cgMLST) are used in routine clinical practice to characterize bacterial isolates, track the spread of infectious diseases, and monitor outbreaks. However, the limitation lies in distinguishing closely related bacterial strains, which differ only in several bases. Therefore, here, we present the core genome methylome analysis utilizing nanopore sequencing. We demonstrate that epigenetic information contained within bacterial strains allows us to differentiate populations similarly to cgMLST. Moreover, the proposed unique combination of cgMLST with core genome methylome can even increase the discriminatory power between closely similar isolates, overcoming the constraints of cgMLST. Combining genomic and epigenomic information can provide better insight into bacterial strains&apos; evolution, transmission patterns and pathogenicity study.

Název v anglickém jazyce

Exploring DNA Methylation Patterns in the Core Genome of Klebsiella pneumoniae

Popis výsledku anglicky

In recent years, sequencing has become easily accessible and widespread, revolutionizing our ability to study complex genetic information. Thus, it is possible to analyze complex bacterial populations and investigate relationships between individual strains. Methods such as core genome multilocus sequence typing (cgMLST) are used in routine clinical practice to characterize bacterial isolates, track the spread of infectious diseases, and monitor outbreaks. However, the limitation lies in distinguishing closely related bacterial strains, which differ only in several bases. Therefore, here, we present the core genome methylome analysis utilizing nanopore sequencing. We demonstrate that epigenetic information contained within bacterial strains allows us to differentiate populations similarly to cgMLST. Moreover, the proposed unique combination of cgMLST with core genome methylome can even increase the discriminatory power between closely similar isolates, overcoming the constraints of cgMLST. Combining genomic and epigenomic information can provide better insight into bacterial strains&apos; evolution, transmission patterns and pathogenicity study.

Druh

D - Stať ve sborníku

CEP obor

—

OECD FORD obor

30200 - Clinical medicine

Projekt

<a href="/cs/project/GA23-05845S" target="_blank" >GA23-05845S: Určování infekčních hrozeb v reálném čase ze surových nanoporových signálů pomocí technik strojového učení</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Bioinformatics and Biomedical Engineering, PT II, IWBBIO 2024

ISBN

978-3-031-64636-2

ISSN

2366-6323

e-ISSN

1611-3349

Počet stran výsledku

13

Strana od-do

140-152

Název nakladatele

SPRINGER INTERNATIONAL PUBLISHING AG

Místo vydání

CHAM

Místo konání akce

Meloneras

Datum konání akce

15. 7. 2024

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

001308622700011