Cyclodextrin glucanotransferase production by cell biocatalysts of alkaliphilic bacilli

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67179843%3A_____%2F09%3A00328089" target="_blank" >RIV/67179843:_____/09:00328089 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cyclodextrin glucanotransferase production by cell biocatalysts of alkaliphilic bacilli

Popis výsledku v původním jazyce

Cells of Bacillus pseudalcaliphilus 20RF and Bacillus pseudalcaliphilus 8SB isolated from Bulgarian habitats, producers of cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19), were immobilized by three different techniques: on two types of polysulphonemembranes; entrapped in agar-gel beads containing magnetite and by nano-particles of silanized magnetite covalently bound on the cell surface. The biocatalysts obtained demonstrated the opportunity for a significantly enhanced CGTase production comparedto free cells for a long period of time (10 days semicontinuous cultivation) without impact on their mechanical stability. The cell membrane-biocatalysts exhibited the highest enzyme aktivity. Membrane biocatalysts were applied for a direct cyclodextrinproduction. The results obtained demonstrated the possibility of starch conversion into cyclodextrins by immobilized cells.

Název v anglickém jazyce

Cyclodextrin glucanotransferase production by cell biocatalysts of alkaliphilic bacilli

Popis výsledku anglicky

Cells of Bacillus pseudalcaliphilus 20RF and Bacillus pseudalcaliphilus 8SB isolated from Bulgarian habitats, producers of cyclodextrin glucanotransferase (CGTase, EC 2.4.1.19), were immobilized by three different techniques: on two types of polysulphonemembranes; entrapped in agar-gel beads containing magnetite and by nano-particles of silanized magnetite covalently bound on the cell surface. The biocatalysts obtained demonstrated the opportunity for a significantly enhanced CGTase production comparedto free cells for a long period of time (10 days semicontinuous cultivation) without impact on their mechanical stability. The cell membrane-biocatalysts exhibited the highest enzyme aktivity. Membrane biocatalysts were applied for a direct cyclodextrinproduction. The results obtained demonstrated the possibility of starch conversion into cyclodextrins by immobilized cells.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochemical Engineering Journal

ISSN

1369-703X

e-ISSN

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Svazek periodika

46

Číslo periodika v rámci svazku

-

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

000274797800006

EID výsledku v databázi Scopus

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