Magnetic particles?based biosensor for biogenic amines using an optical oxygen sensor as a transducer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67179843%3A_____%2F13%3A00388384" target="_blank" >RIV/67179843:_____/13:00388384 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985858:_____/13:00388384 RIV/61989592:15310/13:33147699

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00604-012-0932-0" target="_blank" >http://dx.doi.org/10.1007/s00604-012-0932-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00604-012-0932-0" target="_blank" >10.1007/s00604-012-0932-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Magnetic particles?based biosensor for biogenic amines using an optical oxygen sensor as a transducer

Popis výsledku v původním jazyce

We have developed a fibre optic biosensor with incorporated magnetic microparticles for the determination of biogenic amines. The enzyme diamine oxidase from Pisum sativum was immobilized either on chitosan-coated magnetic microparticles or on commercialmicrobeads modified with a ferrofluid. Both the immobilized enzyme and the ruthenium complex were incorporated into a UV-cured inorganic?organic polymer composite and deposited on a lens that was connected, by optical fibres, to an electro-optical detector. The enzyme catalyzes the oxidation of amines under consumption of oxygen. The latter was determined by measuring the quenched fluorescence lifetime of the ruthenium complex. The limits of detection for the biogenic amines putrescine and cadaverine are 25?30 ?mol?L1, and responses are linear up to a concentration of 1 mmol L1.

Název v anglickém jazyce

Magnetic particles?based biosensor for biogenic amines using an optical oxygen sensor as a transducer

Popis výsledku anglicky

We have developed a fibre optic biosensor with incorporated magnetic microparticles for the determination of biogenic amines. The enzyme diamine oxidase from Pisum sativum was immobilized either on chitosan-coated magnetic microparticles or on commercialmicrobeads modified with a ferrofluid. Both the immobilized enzyme and the ruthenium complex were incorporated into a UV-cured inorganic?organic polymer composite and deposited on a lens that was connected, by optical fibres, to an electro-optical detector. The enzyme catalyzes the oxidation of amines under consumption of oxygen. The latter was determined by measuring the quenched fluorescence lifetime of the ruthenium complex. The limits of detection for the biogenic amines putrescine and cadaverine are 25?30 ?mol?L1, and responses are linear up to a concentration of 1 mmol L1.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EH - Ekologie – společenstva

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microchimica Acta

ISSN

0026-3672

e-ISSN

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Svazek periodika

180

Číslo periodika v rámci svazku

3-4

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

8

Strana od-do

311-318

Kód UT WoS článku

000313837900017

EID výsledku v databázi Scopus

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